| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPSb2 |
| Uniprot No | P20231 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-275aa |
| 氨基酸序列 | IVGGQEAPRSKWPWQVSLRVHGPYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP |
| 预测分子量 | 31.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPSb2重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: *Heterologous Expression and Functional Characterization of Recombinant TPSb2 in E. coli*
**作者**: Zhang L, Wang Y, Chen H
**摘要**: 本研究报道了TPSb2基因在大肠杆菌中的重组表达与纯化。通过优化密码子使用及诱导条件，成功获得可溶性蛋白，并利用亲和层析纯化。功能实验表明，重组TPSb2在体外具有显著的蛋白酶活性，可能参与细胞凋亡调控。
2. **标题**: *Structural Analysis of TPSb2 Recombinant Protein and Its Role in Cancer Signaling Pathways*
**作者**: Kim S, Tanaka R, Gupta P
**摘要**: 通过X射线晶体学解析了TPSb2重组蛋白的三维结构，揭示其独特的激酶样结构域。体外实验证实，TPSb2与PI3K/AKT通路相互作用，可能通过调控磷酸化过程影响肿瘤细胞增殖。
3. **标题**: *Development of a Mammalian Cell System for High-Yield TPSb2 Production*
**作者**: Müller F, Schmidt D, Wagner T
**摘要**: 建立了一种基于HEK293细胞的稳定表达系统，用于高效生产重组TPSb2蛋白。纯化后的蛋白在糖基化修饰下表现出更强的稳定性，并成功应用于小鼠模型的免疫治疗研究。
4. **标题**: *Functional Screening of TPSb2 Mutants Reveals Critical Residues for Substrate Binding*
**作者**: Li X, Zhou Q, Park J
**摘要**: 通过定点突变技术构建了TPSb2的多个突变体，结合表面等离子体共振(SPR)分析，鉴定了其底物结合的关键氨基酸位点，为靶向药物设计提供了结构基础。
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注：以上内容为示例性虚构文献，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。若需具体领域文献，建议补充蛋白全称或研究背景。
TPSb2. a recombinant protein derived from thymic stromal lymphopoietin (TSLP), is a cytokine implicated in immune regulation, particularly in Th2-mediated inflammatory responses. TSLP, initially identified in thymic stromal cells, exists in two major splice variants: a long isoform (TSLP-L) with potent pro-inflammatory activity and a shorter isoform (TSLP-S or TPSb2) exhibiting distinct functional properties. TPSb2 lacks exon 4 of the TSLP gene, resulting in a truncated protein structure that alters receptor binding specificity. Unlike TSLP-L, which activates dendritic cells via the TSLPR-IL-7Rα heterodimer, TPSb2 shows reduced affinity for this receptor complex and may interact with alternative signaling pathways.
Recombinant TPSb2 is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications. Its production enables detailed studies of isoform-specific functions in allergic diseases (e.g., asthma, atopic dermatitis) and autoimmune disorders. Emerging evidence suggests TPSb2 modulates immune responses differently from TSLP-L, potentially acting as a natural regulatory counterpart to balance inflammatory processes. Research highlights its role in epithelial cell communication, eosinophil activation, and crosstalk between innate and adaptive immunity. Pharmaceutical interest focuses on developing TPSb2-based therapies or inhibitors targeting TSLP pathways, with clinical implications for precision immunomodulation. However, its exact physiological role remains under investigation, particularly regarding tissue-specific expression patterns and disease context-dependent behavior.
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