| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPSg1 |
| Uniprot No | Q9NRR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321aa |
| 氨基酸序列 | MALGACGLLLLLAVPGVSLRTLQPGCGRPQVSDAGGRIVGGHAAPAGAWPWQASLRLRRVHVCGGSLLSPQWVLTAAHCFSGSLNSSDYQVHLGELEITLSPHFSTVRQIILHSSPSGQPGTSGDIALVELSVPVTLSSRILPVCLPEASDDFCPGIRCWVTGWGYTREGEPLPPPYSLREVKVSVVDTETCRRDYPGPGGSILQPDMLCARGPGDACQDDSGGPLVCQVNGAWVQAGTVSWGEGCGRPNRPGVYTRVPAYVNWIRRHITASGGSESGYPRLPLLAGFFLPGLFLLLVSCVLLAKCLLHPSADGTPFPAPD |
| 预测分子量 | 33,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPSg1重组蛋白的3篇示例参考文献(注：以下为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Cloning and Functional Expression of Recombinant Human TPSg1 in Mammalian Cells"
**作者**: K. Tanaka, et al.
**摘要**: 该研究成功克隆了人源TPSg1基因，并在哺乳动物细胞系中实现了重组表达。通过Western blot和酶活性检测证实了表达蛋白的功能性，为后续药理研究提供了工具。
2. **文献名称**: "Structural Characterization of Recombinant TPSg1 Using X-ray Crystallography"
**作者**: M. Johnson & R. Lee
**摘要**: 本文报道了通过大肠杆菌表达系统纯化的TPSg1重组蛋白的晶体结构解析，揭示了其活性位点的关键氨基酸残基，为靶向抑制剂设计提供了结构基础。
3. **文献名称**: "TPSg1 Recombinant Protein Production in Yeast: Optimization and Biomedical Applications"
**作者**: S. Patel, et al.
**摘要**: 研究利用毕赤酵母系统高效表达TPSg1重组蛋白，优化了发酵条件以提高产量，并验证其在体外血栓形成模型中的调控作用，展示了潜在的治疗应用价值。
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建议通过PubMed、Google Scholar等平台以关键词“TPSg1 recombinant protein”或“Thromboxane Synthase 1 expression”检索最新文献以获取准确信息。
**Background of TPSg1 Recombinant Protein**
TPSg1 (Thrombospondin type-1 domain-containing protein 1) is a recombinant protein derived from the *TSPG1* gene, which encodes a member of the thrombospondin protein family. Thrombospondins are multifunctional extracellular matrix glycoproteins involved in cell-matrix interactions, angiogenesis, and tissue remodeling. The TPSg1 protein contains a conserved thrombospondin type-1 repeat (TSR) domain, a hallmark of this family, which mediates interactions with cytokines, receptors, and other extracellular molecules.
The *TSPG1* gene is expressed in various tissues, including the nervous system, cardiovascular system, and connective tissues, suggesting roles in neural development, vascular homeostasis, and wound healing. Studies indicate that TPSg1 may regulate cell adhesion, migration, and signaling pathways, particularly through interactions with integrins or growth factors like TGF-β. Dysregulation of TPSg1 has been linked to pathological conditions, such as tumor progression, fibrosis, and neurodegenerative diseases, highlighting its potential as a therapeutic or diagnostic target.
Recombinant TPSg1 is typically produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications for functional studies. Its applications span structural biology, drug screening, and mechanistic research to elucidate its role in health and disease. By studying TPSg1. researchers aim to uncover novel strategies for modulating extracellular matrix dynamics or targeting pathways implicated in cancer and tissue fibrosis.
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