| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCg2 |
| Uniprot No | P16885 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1265aa |
| 氨基酸序列 | MSTTVNVDSLAEYEKSQIKRALELGTVMTVFSFRKSTPERRTVQVIMETRQVAWSKTADKIEGFLDIMEIKEIRPGKNSKDFERAKAVRQKEDCCFTILYGTQFVLSTLSLAADSKEDAVNWLSGLKILHQEAMNASTPTIIESWLRKQIYSVDQTRRNSISLRELKTILPLINFKVSSAKFLKDKFVEIGAHKDELSFEQFHLFYKKLMFEQQKSILDEFKKDSSVFILGNTDRPDASAVYLHDFQRFLIHEQQEHWAQDLNKVRERMTKFIDDTMRETAEPFLFVDEFLTYLFSRENSIWDEKYDAVDMQDMNNPLSHYWISSSHNTYLTGDQLRSESSPEAYIRCLRMGCRCIELDCWDGPDGKPVIYHGWTRTTKIKFDDVVQAIKDHAFVTSSFPVILSIEEHCSVEQQRHMAKAFKEVFGDLLLTKPTEASADQLPSPSQLREKIIIKHKKLGPRGDVDVNMEDKKDEHKQQGELYMWDSIDQKWTRHYCAIADAKLSFSDDIEQTMEEEVPQDIPPTELHFGEKWFHKKVEKRTSAEKLLQEYCMETGGKDGTFLVRESETFPNDYTLSFWRSGRVQHCRIRSTMEGGTLKYYLTDNLTFSSIYALIQHYRETHLRCAEFELRLTDPVPNPNPHESKPWYYDSLSRGEAEDMLMRIPRDGAFLIRKREGSDSYAITFRARGKVKHCRINRDGRHFVLGTSAYFESLVELVSYYEKHSLYRKMRLRYPVTPELLERYNMERDINSLYDVSRMYVDPSEINPSMPQRTVKALYDYKAKRSDELSFCRGALIHNVSKEPGGWWKGDYGTRIQQYFPSNYVEDISTADFEELEKQIIEDNPLGSLCRGILDLNTYNVVKAPQGKNQKSFVFILEPKQQGDPPVEFATDRVEELFEWFQSIREITWKIDTKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPVDLLKYNQKGLTRVYPKGQRVDSSNYDPFRLWLCGSQMVALNFQTADKYMQMNHALFSLNGRTGYVLQPESMRTEKYDPMPPESQRKILMTLTVKVLGARHLPKLGRSIACPFVEVEICGAEYDNNKFKTTVVNDNGLSPIWAPTQEKVTFEIYDPNLAFLRFVVYEEDMFSDPNFLAHATYPIKAVKSGFRSVPLKNGYSEDIELASLLVFCEMRPVLESEEELYSSCRQLRRRQEELNNQLFLYDTHQNLRNANRDALVKEFSVNENQLQLYQEKCNKRLREKRVSNSKFYS |
| 预测分子量 | 147,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLCγ2重组蛋白的参考文献信息(文献标题及摘要为简化概述)：
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1. **标题**: "Structural insights into the regulation of human phospholipase Cγ2"
**作者**: S. Bunney et al.
**摘要**: 通过X射线晶体学解析人源PLCγ2重组蛋白的结构，揭示其自抑制机制及被酪氨酸激酶激活的关键构象变化，为免疫受体信号传导提供分子基础。
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2. **标题**: "PLCγ2 regulates B cell receptor signaling via dynamic membrane interactions"
**作者**: K. Deason et al.
**摘要**: 利用重组PLCγ2蛋白研究其在B细胞受体信号中的作用，发现其通过PH结构域动态结合细胞膜脂质，调控下游钙离子信号及细胞活化。
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3. **标题**: "A novel autoinflammatory disorder caused by PLCG2 mutations: functional characterization of recombinant mutant proteins"
**作者**: Q. Zhou et al.
**摘要**: 构建携带PLCG2基因突变的重组蛋白，揭示突变导致酶活性异常增强，与自身炎症性疾病APLAID综合征的发病机制直接相关。
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提示：以上文献年份及作者为示例，实际引用时建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)搜索最新研究。
Phospholipase C gamma 2 (PLCγ2) is a critical enzyme in intracellular signaling, primarily involved in immune cell regulation. As a member of the phospholipase C family, it hydrolyzes phosphatidylinositol 4.5-bisphosphate (PIP2) into secondary messengers inositol 1.4.5-trisphosphate (IP3) and diacylglycerol (DAG), which mediate calcium release and protein kinase C activation. PLCγ2 is highly expressed in hematopoietic cells, particularly B lymphocytes, mast cells, and platelets, where it plays a pivotal role in B-cell receptor (BCR) signaling, Fc receptor activation, and innate immune responses. Dysregulation of PLCγ2 is linked to immune disorders, such as common variable immunodeficiency (CVID), and gain-of-function mutations are associated with autoinflammatory diseases.
Recombinant PLCγ2 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional PLCγ2 for research and therapeutic applications. Its recombinant form retains enzymatic activity and structural domains, including the pleckstrin homology (PH) domain, split catalytic core, and SH2/SH3 adaptor regions, enabling studies on signaling mechanisms, drug screening, and disease modeling. Researchers utilize it to investigate immune dysregulation, develop inhibitors for hyperactive PLCγ2 variants, or restore signaling in deficiency models. Its production often includes tags (e.g., His-tag) for purification and detection, ensuring high specificity in assays. As a tool, recombinant PLCγ2 bridges molecular insights and translational applications in immunology and precision medicine.
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