| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P4Ha3 |
| Uniprot No | Q7Z4N8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-544aa |
| 氨基酸序列 | DPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRPPYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED |
| 预测分子量 | 75.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P4Ha3重组蛋白的模拟参考文献示例，供参考：
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1. **文献名称**: *Recombinant P4Ha3 facilitates collagen hydroxylation in vitro: Implications for fibrosis therapy*
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究成功表达并纯化了重组P4Ha3蛋白，验证其在体外对胶原脯氨酸残基的羟化活性。实验表明，P4Ha3的酶活依赖性与其在纤维化组织中的高表达相关，提示其作为抗纤维化治疗靶点的潜力。
2. **文献名称**: *Structural insights into the catalytic mechanism of P4Ha3 through cryo-EM analysis*
**作者**: Tanaka K, Suzuki H, et al.
**摘要**: 通过冷冻电镜解析了重组P4Ha3蛋白的三维结构，揭示了其α-酮戊二酸结合域的关键氨基酸位点，阐明了羟化反应的分子机制，为设计选择性抑制剂提供结构基础。
3. **文献名称**: *P4Ha3 regulates tumor collagen crosslinking and metastasis in a murine model*
**作者**: Rodriguez S, Patel R, et al.
**摘要**: 利用重组P4Ha3蛋白干预肿瘤微环境，发现其通过增强胶原交联密度抑制癌细胞侵袭。研究揭示了P4Ha3在肿瘤转移中的双重调控作用，为癌症治疗提供新思路。
4. **文献名称**: *A high-throughput assay for screening P4Ha3 modulators using recombinant protein*
**作者**: Gupta M, Lee T, et al.
**摘要**: 开发基于重组P4Ha3蛋白的高通量筛选平台，用于快速检测小分子化合物对其酶活的抑制或激活效应，为药物开发提供高效工具。
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**说明**：以上文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。若需文献检索方法指导，可进一步说明需求。
**Background of P4Ha3 Recombinant Protein**
Prolyl 4-hydroxylase subunit alpha 3 (P4Ha3), encoded by the *P4HA3* gene, is a catalytic subunit of the prolyl 4-hydroxylase family. This enzyme plays a critical role in post-translational modification of collagen, specifically hydroxylating proline residues in collagen chains—a process essential for stabilizing collagen’s triple-helical structure. Collagen, a major component of the extracellular matrix (ECM), provides structural support to tissues, and its proper maturation relies on P4Ha3 activity. Unlike other isoforms (e.g., P4Ha1/2), P4Ha3 exhibits distinct tissue-specific expression patterns, with higher levels observed in tissues requiring rapid collagen turnover, such as bone, cartilage, and certain cancers.
Recombinant P4Ha3 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce functional enzyme for research and therapeutic applications. Its production often involves co-expression with the beta subunit (P4HB) to reconstitute active enzyme complexes. Purification techniques, such as affinity chromatography, ensure high purity and activity.
Studies highlight P4Ha3’s involvement in pathological conditions, including fibrosis, tumor metastasis, and skeletal disorders. Overexpression in cancers correlates with enhanced ECM remodeling, promoting invasion and angiogenesis. Conversely, P4Ha3 dysregulation is linked to collagen-related defects, such as osteogenesis imperfecta. Researchers utilize recombinant P4Ha3 to study collagen biochemistry, screen inhibitors for antifibrotic therapies, and model diseases. Challenges remain in understanding isoform-specific roles and developing selective modulators. Current efforts focus on structural analysis of P4Ha3-substrate interactions and optimizing recombinant production for biomedical applications, including tissue engineering and regenerative medicine.
This recombinant tool bridges fundamental collagen biology to translational research, offering insights into ECM dynamics and therapeutic targeting.
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