| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCSK5 |
| Uniprot No | Q92824 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PCSK5重组蛋白的虚构参考文献示例(基于领域研究逻辑合理虚构，实际文献需通过学术数据库检索验证)：
1. **《Structural characterization of recombinant human PCSK5 protease domain》**
- **作者**: Zhang L, et al. (2020)
- **摘要**: 本研究通过大肠杆菌表达系统获得重组人源PCSK5蛋白酶结构域，利用X射线晶体学解析其三维结构，揭示了其催化活性位点与底物结合口袋的构象特征，为开发靶向抑制剂提供结构基础。
2. **《Functional analysis of PCSK5 in TGF-β precursor processing》**
- **作者**: Smith JR, et al. (2018)
- **摘要**: 通过昆虫细胞表达系统制备重组PCSK5蛋白，证实其在体外可切割TGF-β前体蛋白的特定位点，并通过基因敲除实验证明PCSK5缺失导致细胞分化异常，提示其在生长因子调控中的关键作用。
3. **《Role of PCSK5 in cardiovascular disease: Insights from recombinant protein assays》**
- **作者**: Gupta S, et al. (2021)
- **摘要**: 利用哺乳动物细胞表达的高纯度PCSK5重组蛋白进行功能研究，发现其与低密度脂蛋白受体(LDLR)存在直接互作，可能通过非经典途径影响血脂代谢，为动脉粥样硬化发病机制提供新视角。
注：以上文献信息为模拟生成，实际研究中建议通过PubMed、Web of Science等平台以关键词"PCSK5 recombinant protein"或"proprotein convertase 5"检索最新实证文献。
**Background of PCSK5 Recombinant Protein**
PCSK5 (Proprotein Convertase Subtilisin/Kexin Type 5), also known as PC5/6. is a member of the proprotein convertase family of serine endoproteases. These enzymes play critical roles in cleaving inactive precursor proteins into biologically active forms, regulating diverse physiological processes such as hormone maturation, growth factor activation, and cellular signaling. PCSK5 exists in two isoforms: a secreted form (PC5/6A) and a transmembrane-bound form (PC5/6B), each exhibiting distinct tissue distribution and substrate specificities. It is widely expressed in tissues, including the brain, intestine, and reproductive organs, and is implicated in embryonic development, lipid metabolism, and immune responses.
Recombinant PCSK5 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications, particularly glycosylation, which is essential for its enzymatic activity. This recombinant form allows researchers to study its substrate cleavage mechanisms, structure-function relationships, and interactions with inhibitors or co-factors *in vitro*. Notably, PCSK5 has gained attention for its role in activating proteins involved in viral entry (e.g., SARS-CoV-2 spike protein) and its association with diseases such as cancer, atherosclerosis, and metabolic disorders.
Studies using recombinant PCSK5 have highlighted its potential as a therapeutic target. Inhibitors or modulators of PCSK5 activity could offer novel strategies for treating conditions linked to dysregulated proteolytic processing. Furthermore, its involvement in developmental pathways underscores its importance in basic research. By leveraging recombinant PCSK5. scientists aim to unravel its physiological and pathological roles, paving the way for diagnostic or therapeutic innovations.
×
