| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GKN2 |
| Uniprot No | Q86XP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-184aa |
| 氨基酸序列 | YEVFNIISPS NNGGNVQETV TIDNEKNTAI INIHAGSCSS TTIFDYKHGY IASRVLSRRA CFILKMDHQN IPPLNNLQWY IYEKQALDNM FSSKYTWVKY NPLESLIKDV DWFLLGSPIE KLCKHIPLYK GEVVENTHNV GAGGCAKAGL LGILGISICA DIHV |
| 预测分子量 | 20,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GKN2重组蛋白的3篇参考文献示例(注：部分信息可能需通过学术数据库验证)：
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1. **文献名称**: *"Recombinant human gastrokine 2 protein inhibits cell proliferation and migration in gastric cancer"*
**作者**: Zhang Y, et al. (2015)
**摘要**: 研究通过大肠杆菌表达系统成功制备重组人GKN2蛋白，发现其可抑制胃癌细胞增殖和迁移，可能通过调控EGFR/AKT信号通路发挥作用。
2. **文献名称**: *"Expression and purification of bioactive recombinant gastrokine-2 in Pichia pastoris"*
**作者**: Lee S, et al. (2017)
**摘要**: 利用毕赤酵母表达系统高效表达功能性重组GKN2蛋白，纯化产物在体外实验中显示出对胃黏膜炎症反应的保护作用。
3. **文献名称**: *"GKN2重组蛋白通过调控自噬抑制幽门螺杆菌诱导的胃上皮细胞损伤"*
**作者**: 王X等 (2020)
**摘要**: 研究证实重组GKN2蛋白可通过激活自噬通路减轻幽门螺杆菌引起的胃上皮细胞氧化应激和凋亡，为其治疗应用提供依据。
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**说明**：以上文献为示例，实际引用时建议通过PubMed、Web of Science或CNKI等平台核实具体信息。GKN2相关研究多聚焦于其在胃癌、黏膜修复及幽门螺杆菌感染中的功能机制。
**Background of GKN2 Recombinant Protein**
Gastrokine-2 (GKN2), a secreted protein predominantly expressed in the gastric mucosa, plays a critical role in maintaining gastrointestinal homeostasis. It belongs to the gastrokine family, which includes GKN1 and GKN3. and is specifically localized in the superficial epithelial cells of the stomach. GKN2 is characterized by a conserved N-terminal BRICHOS domain and a C-terminal trefoil domain, structural motifs associated with mucosal repair and cytoprotection. Functionally, GKN2 contributes to the integrity of the gastric mucosal barrier by regulating cell proliferation, differentiation, and apoptosis, while also modulating inflammatory responses and oxidative stress.
Studies highlight its involvement in gastric pathophysiology. Downregulation or loss of GKN2 expression is strongly linked to gastric carcinogenesis, where it may act as a tumor suppressor by inhibiting epithelial-mesenchymal transition (EMT) and metastasis. Additionally, GKN2 interacts with tumor-associated proteins like TFF1. further underscoring its role in gastric cancer progression. Its recombinant form, produced via bacterial or eukaryotic expression systems, retains bioactive properties, enabling researchers to explore its mechanisms in vitro and in vivo.
Therapeutic potential of recombinant GKN2 is emerging, particularly in gastric disease models, where it demonstrates anti-inflammatory, antioxidant, and anti-tumor effects. Its recombinant variant is also utilized as a biomarker for early gastric cancer detection. Ongoing research aims to harness its protective and regulatory functions for novel diagnostic and therapeutic strategies in gastrointestinal disorders.
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