| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD45 |
| Uniprot No | P08575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-577aa |
| 氨基酸序列 | QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK |
| 预测分子量 | 76.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD45重组蛋白的示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and Purification of Functional CD45 Cytoplasmic Domain Using a Bacterial System*
**作者**：Smith A, et al.
**摘要**：该研究报道了利用大肠杆菌表达系统克隆并纯化CD45胞内结构域重组蛋白，通过体外磷酸酶活性实验证实其功能，为后续结构-功能研究提供基础材料。
2. **文献名称**：*Structural Insights into CD45 Receptor Phosphatase by Cryo-EM Using Recombinant Protein*
**作者**：Johnson R, et al.
**摘要**：通过昆虫细胞表达系统获得全长CD45重组蛋白，利用冷冻电镜解析其三维结构，揭示了其调控免疫受体信号传导的分子机制。
3. **文献名称**：*Kinetic Analysis of CD45 Recombinant Protein in T-cell Signaling Modulation*
**作者**：Brown K, et al.
**摘要**：研究采用哺乳动物细胞表达的重组CD45蛋白，在体外验证其通过去磷酸化作用负向调控T细胞受体(TCR)信号通路的关键功能。
4. **文献名称**：*Application of Recombinant CD45 in High-throughput Drug Screening for Autoimmune Diseases*
**作者**：Lee M, et al.
**摘要**：开发了基于CD45重组蛋白的高通量抑制剂筛选平台，用于发现靶向其磷酸酶活性的潜在自身免疫疾病治疗药物。
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建议通过PubMed或Google Scholar搜索关键词“CD45 recombinant protein expression”、“CD45 phosphatase structure”等获取具体文献。
CD45. also known as leukocyte common antigen (LCA), is a transmembrane protein tyrosine phosphatase (PTP) expressed on all nucleated hematopoietic cells, including T cells, B cells, and myeloid cells. It plays a pivotal role in regulating immune cell activation and signaling by modulating the activity of Src-family kinases (SFKs) through dephosphorylation of their inhibitory C-terminal tyrosine residues. Structurally, CD45 consists of a heavily glycosylated extracellular domain, a single transmembrane region, and two intracellular phosphatase domains. Alternative splicing of exons 4-6 generates multiple isoforms differing in extracellular domains, which influence ligand interactions and cellular responses.
Recombinant CD45 proteins are engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to study its biochemical functions and therapeutic potential. These proteins retain enzymatic activity and are critical tools for investigating immune signaling pathways, particularly in T-cell receptor (TCR) and B-cell receptor (BCR) activation. Researchers use recombinant CD45 to dissect its role in autoimmune diseases, immunodeficiency disorders, and cancer, where dysregulated CD45 expression or mutations correlate with pathological states. For example, CD45-deficient models highlight its importance in lymphocyte development, while aberrant splicing is linked to multiple sclerosis and leukemia.
In drug discovery, recombinant CD45 aids in screening inhibitors targeting its phosphatase activity, offering potential for immunomodulatory therapies. Its extracellular domain is also explored for diagnostic applications, such as detecting soluble CD45 in inflammatory conditions. Despite challenges in crystallizing full-length CD45 due to its size and glycosylation, recombinant fragments have advanced structural studies, revealing mechanisms underlying substrate specificity and regulation. Overall, CD45 recombinant proteins remain indispensable for elucidating immune regulation and developing targeted interventions in hematologic and immune-mediated diseases.
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