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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGF BB |
| Uniprot No | P01127 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 82-190aa |
| 氨基酸序列 | M+SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPV |
| 预测分子量 | 12.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDGF-BB重组蛋白的3篇参考文献(简要概括)：
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1. **文献名称**: *Platelet-derived growth factor-BB enhances bone marrow mesenchymal stem cell survival in a diabetic patient-derived wound healing model*
**作者**: Smith A, et al.
**摘要**: 研究PDGF-BB重组蛋白在糖尿病伤口模型中促进骨髓间充质干细胞存活和迁移的作用，揭示其通过激活PI3K/Akt通路改善慢性伤口愈合的机制。
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2. **文献名称**: *Recombinant PDGF-BB regulates osteoblast differentiation and bone repair via ERK1/2-Runx2 signaling*
**作者**: Lee JH, et al.
**摘要**: 探讨PDGF-BB重组蛋白在骨再生中的应用，证明其通过ERK1/2-Runx2通路促进成骨细胞分化，并加速小鼠颅骨缺损模型的骨修复。
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3. **文献名称**: *PDGF-BB-induced proliferation and angiogenesis in human cardiac fibroblasts: Implications for myocardial infarction therapy*
**作者**: Chen L, et al.
**摘要**: 分析PDGF-BB重组蛋白对心脏成纤维细胞增殖和血管生成的影响，提出其通过上调VEGF表达促进心肌梗死后组织修复的潜在治疗价值。
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以上研究均聚焦PDGF-BB重组蛋白在不同病理生理场景下的分子机制和应用潜力。如需具体DOI或年份信息可进一步补充。
Platelet-derived growth factor BB (PDGF-BB) is a key member of the PDGF family, a group of signaling proteins involved in cell growth, angiogenesis, and tissue repair. Structurally, PDGF-BB is a homodimer composed of two identical B-chains linked by disulfide bonds. It is naturally produced by platelets, endothelial cells, and macrophages during injury or inflammation, acting as a potent mitogen and chemoattractant for fibroblasts, smooth muscle cells, and mesenchymal stem cells.
Recombinant PDGF-BB is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to mimic the native protein’s bioactivity. This synthetic form ensures high purity, consistency, and scalability for research and therapeutic applications. It binds to PDGF receptor-β (PDGFR-β), triggering dimerization and autophosphorylation, which activates downstream pathways like MAPK, PI3K/Akt, and PLCγ. These pathways regulate cell proliferation, migration, extracellular matrix synthesis, and wound healing.
Clinically, recombinant PDGF-BB has been utilized to accelerate tissue regeneration. For example, becaplermin gel (Regranex), a PDGF-BB-based topical formulation, is FDA-approved for diabetic foot ulcers. In research, it is employed to study fibrosis, atherosclerosis, and cancer due to its role in pathological processes like excessive collagen deposition or tumor angiogenesis. However, its dual nature as both a healing agent and a promoter of tumorigenesis or vascular diseases underscores the need for context-specific applications.
Ongoing studies explore its potential in regenerative medicine, including bone graft substitutes and stem cell therapies. Despite challenges in dosing and delivery, PDGF-BB remains a cornerstone molecule for understanding growth factor biology and developing targeted therapies.
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