| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AD |
| Uniprot No | P20671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKAKGK |
| 预测分子量 | 14,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于HIST1H2AD重组蛋白的参考文献示例(注：内容为虚构，仅用于格式参考)：
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1. **"HIST1H2AD regulates chromatin dynamics in breast cancer cells"**
*Author: Zhang L. et al. (2020)*
*摘要：* 研究揭示了HIST1H2AD重组蛋白通过改变核小体稳定性影响乳腺癌细胞中雌激素受体(ER)靶基因的转录，提示其作为表观遗传治疗靶点的潜力。
2. **"Structural characterization of recombinant HIST1H2AD and its post-translational modifications"**
*Author: Kimura H. et al. (2018)*
*摘要：* 利用X射线晶体学解析HIST1H2AD重组蛋白的三维结构，发现其C端磷酸化修饰在DNA损伤修复中起关键作用。
3. **"HIST1H2AD knockdown impairs embryonic stem cell differentiation via dysregulation of pluripotency genes"**
*Author: Patel R. & Smith C. (2019)*
*摘要：* 通过CRISPR干扰技术证明HIST1H2AD缺失导致胚胎干细胞分化异常，强调其在维持染色质开放状态中的功能。
4. **"A novel method for high-yield production of recombinant HIST1H2AD in E. coli"**
*Author: González F. et al. (2021)*
*摘要：* 开发了一种基于大肠杆菌表达系统的重组HIST1H2AD高效纯化方案，解决了组蛋白类蛋白易沉淀的技术难题。
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提示：实际文献需通过PubMed/Google Scholar检索关键词(如"HIST1H2AD recombinant"+"function")。若需真实文献，可补充具体研究方向(如癌症、表观遗传学应用等)。
The HIST1H2AD recombinant protein is derived from the HIST1H2AD gene, which encodes a member of the histone H2A family. Histones are essential nuclear proteins that organize DNA into nucleosomes, the fundamental units of chromatin structure. As a core component of the nucleosome, H2A histones interact with H2B, H3. and H4 to form an octamer around which DNA is wrapped, ensuring genome compaction and regulating DNA accessibility for processes like transcription, replication, and repair. HIST1H2AD, specifically, belongs to the HIST1 cluster on chromosome 6 and is a replication-dependent histone involved in maintaining chromatin integrity during cell division.
Recombinant HIST1H2AD is produced in vitro using expression systems (e.g., E. coli or mammalian cells) to generate a purified, biologically active protein for research applications. It typically retains post-translational modification sites critical for studying epigenetic regulation, such as phosphorylation, ubiquitination, or acetylation, which influence chromatin dynamics and gene expression. Researchers utilize this recombinant protein to investigate nucleosome assembly, histone-DNA interactions, and the role of H2A variants in diseases like cancer, where histone mutations or dysregulation are linked to genomic instability. It also serves as a tool for developing assays to screen epigenetic drugs or study chromatin remodeling complexes. Its production enables standardized, scalable studies into histone function, advancing our understanding of epigenetics and potential therapeutic targets.
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