| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCAF11 |
| Uniprot No | Q99590 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1463aa |
| 氨基酸序列 | MKKKTVCTLNMGDKKYEDMEGEENGDNTISTGLLYSEADRCPICLNCLLEKEVGFPESCNHVFCMTCILKWAETLASCPIDRKPFQAVFKFSALEGYVKVQVKKQLRETKDKKNENSFEKQVSCHENSKSCIRRKAIVREDLLSAKVCDLKWIHRNSLYSETGGKKNAAIKINKPQRSNWSTNQCFRNFFSNMFSSVSHSGESSFTYRAYCTEFIEASEISALIRQKRHELELSWFPDTLPGIGRIGFIPWNVETEVLPLISSVLPRTIFPTSTISFEHFGTSCKGYALAHTQEGEEKKQTSGTSNTRGSRRKPAMTTPTRRSTRNTRAETASQSQRSPISDNSGCDAPGNSNPSLSVPSSAESEKQTRQAPKRKSVRRGRKPPLLKKKLRSSVAAPEKSSSNDSVDEETAESDTSPVLEKEHQPDVDSSNICTVQTHVENQSANCLKSCNEQIEESEKHTANYDTEERVGSSSSESCAQDLPVLVGEEGEVKKLENTGIEANVLCLESEISENILEKGGDPLEKQDQISGLSQSEVKTDVCTVHLPNDFPTCLTSESKVYQPVSCPLSDLSENVESVVNEEKITESSLVEITEHKDFTLKTEELIESPKLESSEGEIIQTVDRQSVKSPEVQLLGHVETEDVEIIATCDTFGNEDFNNIQDSENNLLKNNLLNTKLEKSLEEKNESLTEHPRSTELPKTHIEQIQKHFSEDNNEMIPMECDSFCSDQNESEVEPSVNADLKQMNENSVTHCSENNMPSSDLADEKVETVSQPSESPKDTIDKTKKPRTRRSRFHSPSTTWSPNKDTPQEKKRPQSPSPRRETGKESRKSQSPSPKNESARGRKKSRSQSPKKDIARERRQSQSRSPKRDTTRESRRSESLSPRRETSRENKRSQPRVKDSSPGEKSRSQSRERESDRDGQRRERERRTRKWSRSRSHSRSPSRCRTKSKSSSFGRIDRDSYSPRWKGRWANDGWRCPRGNDRYRKNDPEKQNENTRKEKNDIHLDADDPNSADKHRNDCPNWITEKINSGPDPRTRNPEKLKESHWEENRNENSGNSWNKNFGSGWVSNRGRGRGNRGRGTYRSSFAYKDQNENRWQNRKPLSGNSNSSGSESFKFVEQQSYKRKSEQEFSFDTPADRSGWTSASSWAVRKTLPADVQNYYSRRGRNSSGPQSGWMKQEEETSGQDSSLKDQTNQQVDGSQLPINMMQPQMNVMQQQMNAQHQPMNIFPYPVGVHAPLMNIQRNPFNIHPQLPLHLHTGVPLMQVATPTSVSQGLPPPPPPPPPSQQVNYIASQPDGKQLQGIPSSSHVSNNMSTPVLPAPTAAPGNTGMVQGPSSGNTSSSSHSKASNAAVKLAESKVSVAVEASADSSKTDKKLQIQEKAAQEVKLAIKPFYQNKDITKEEYKEIVRKAVDKVCHSKSGEVNSTKVANLVKAYVDKYKYSRKGSQKKTLEEPVSTEKNIG |
| 预测分子量 | 164,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCAF11重组蛋白的3篇参考文献示例(注：文献为虚构示例，仅用于演示格式)：
1. **文献名称**: "Expression and functional analysis of recombinant SCAF11 in RNA splicing regulation"
**作者**: Zhang L, et al.
**摘要**: 研究报道了人源SCAF11重组蛋白在大肠杆菌中的高效表达及纯化，并验证其通过CTD结构域与RNA聚合酶II相互作用，调控前体mRNA剪接的分子机制。
2. **文献名称**: "Structural characterization of SCAF11's SR-rich domain using recombinant protein"
**作者**: Müller S, et al.
**摘要**: 通过X射线晶体学解析了SCAF11重组蛋白SR结构域的三维结构，揭示了该区域在介导剪接体组装中的关键磷酸化位点和蛋白互作界面。
3. **文献名称**: "SCAF11 knockout cell lines rescued by recombinant protein show altered alternative splicing patterns"
**作者**: Kim JH, et al.
**摘要**: 利用重组SCAF11蛋白回补基因敲除细胞，证明其恢复SMN2基因的选择性剪接功能，为脊髓性肌萎缩症研究提供实验依据。
SCAF11 (SR-related and CTD-associated factor 11) is a multifunctional protein involved in RNA processing, transcription regulation, and genome stability. Belonging to the SR protein family, it contains characteristic arginine-serine-rich (RS) domains and RNA recognition motifs (RRMs), enabling interactions with RNA, DNA, and protein partners. It binds to the C-terminal domain (CTD) of RNA polymerase II, linking transcription with pre-mRNA splicing by recruiting spliceosomal components. SCAF11 also participates in DNA damage response, resolving R-loops to prevent replication stress, and regulates alternative splicing events impacting cellular processes like apoptosis and cell cycle progression. Dysregulation of SCAF11 is associated with cancers (e.g., leukemia, glioblastoma) and neurological disorders, with studies suggesting roles in tumorigenesis and neurodegeneration. Recombinant SCAF11 proteins, typically produced in bacterial or mammalian systems, are used to study its molecular mechanisms, screen therapeutic compounds, and develop diagnostic tools. Its dual functions in transcription-coupled splicing and genome maintenance make it a compelling target for understanding gene expression regulation and disease pathology.
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