| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADRb1 |
| Uniprot No | P08588 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477aa |
| 氨基酸序列 | MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGATPPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV |
| 预测分子量 | 52.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADRB1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：Crystal Structure of the Human β1-Adrenergic G Protein-Coupled Receptor
**作者**：Warne, T. et al.
**摘要**：本研究首次报道了人源ADRB1重组蛋白的晶体结构，利用昆虫细胞表达系统表达并纯化受体，结合反向激动剂carazolol进行结构解析。揭示了ADRB1跨膜结构域的构象特征及配体结合位点，为理解β1受体信号传导机制提供了结构基础。
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2. **文献名称**：Efficient Expression and Purification of Recombinant β1-Adrenergic Receptor for Structural Studies
**作者**：Tate, C.G. et al.
**摘要**：开发了一种高效的ADRB1重组蛋白哺乳动物细胞表达系统，通过引入热稳定突变和亲和标签优化纯化流程。获得高纯度受体蛋白，适用于X射线晶体学和冷冻电镜研究，推动针对ADRB1的药物设计。
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3. **文献名称**：Directed Evolution of APJ/β1-Adrenergic Receptor Chimeras for Enhanced Stability and Ligand Binding
**作者**：Zhang, X. et al.
**摘要**：通过构建ADRB1与APJ受体的嵌合体，结合定向进化技术提高重组蛋白的稳定性和配体结合能力。研究证实改造后的重组ADRB1在体外保持功能活性，为高通量药物筛选提供了可靠工具。
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**备注**：上述文献为示例，实际文献需通过PubMed或Google Scholar以关键词“ADRB1 recombinant protein”“beta1-adrenergic receptor structure”检索核实。Kobilka、Tate及Lefkowitz等团队的研究可作为重点参考方向。
ADRb1 (Adrenoceptor Beta 1) recombinant protein is a biologically active protein engineered to mimic the native β1-adrenergic receptor, a critical G protein-coupled receptor (GPCR) found predominantly in cardiac tissue. As a key regulator of the sympathetic nervous system, ADRb1 mediates responses to catecholamines like adrenaline and noradrenaline, influencing heart rate, myocardial contractility, and blood pressure. Its recombinant form is synthesized using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and ligand-binding functionality.
Research on ADRb1 recombinant protein is driven by its clinical relevance. Polymorphisms in the ADRb1 gene correlate with cardiovascular disease susceptibility and drug response variability. β1-selective blockers (e.g., metoprolol), widely used for hypertension and heart failure, target this receptor. The recombinant protein enables structural studies (e.g., X-ray crystallography) to map ligand-binding domains and activation mechanisms, aiding in designing improved therapeutics. It also facilitates high-throughput screening for novel agonists/antagonists and investigation of receptor desensitization pathways linked to heart failure progression.
Purified ADRb1 recombinant protein typically includes tags (e.g., His-tag) for isolation and may be stabilized in micelles or liposomes to maintain conformational integrity. Recent studies focus on biased signaling—how specific ligands preferentially activate G-protein or β-arrestin pathways—to develop safer drugs with fewer side effects. Its applications extend to antibody production for diagnostic assays and exploring cross-talk with other signaling molecules in cardiovascular pathophysiology. As personalized medicine advances, ADRb1 recombinant tools remain vital for understanding interindividual treatment responses and optimizing targeted therapies.
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