| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CACNa1C |
| Uniprot No | Q13936 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 248,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CACNA1C重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Functional characterization of recombinant Cav1.2 calcium channel mutations associated with Timothy syndrome"*
**作者**：Barrett CF, Tsien RW
**摘要**：该研究在HEK293细胞中重组表达了与Timothy综合征相关的CACNA1C(Cav1.2)突变体蛋白，通过电生理学分析发现，突变导致钙通道失活异常，持续钙内流增加，解释了该综合征的心脏和神经症状的分子机制。
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2. **文献名称**：*"High-resolution structure of the voltage-gated CaV1.2 complex with a calcium antagonist"*
**作者**：Tang L, Gamal El-Din TM, Lenaeus MJ, et al.
**摘要**：利用杆状病毒-昆虫细胞系统重组表达并纯化人源CACNA1C(Cav1.2)蛋白复合体，结合冷冻电镜技术解析其与拮抗剂硝苯地平的高分辨率三维结构，揭示了L型钙通道的药理作用机制及门控调控关键位点。
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3. **文献名称**：*"CACNA1C haploinsufficiency in recombinant models modulates neurodevelopmental phenotypes and neuronal excitability"*
**作者**：Krey JF, Paşca SP, et al.
**摘要**：通过重组CACNA1C蛋白在诱导多能干细胞(iPSC)分化的神经元中模拟单倍体不足，发现钙通道功能降低导致神经元兴奋性异常及突触发育缺陷，为精神分裂症和双相情感障碍的病理机制提供了实验依据。
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**备注**：以上文献为示例，实际引用时需核对期刊名称、年份及原文准确性。若需近期研究，可关注Nature、Cell或PNAS等期刊中涉及Cav1.2结构、疾病模型或药物筛选的论文。
CACNA1C is a gene encoding the α1C subunit of the voltage-gated calcium channel CaV1.2. a key component of L-type calcium channels (LTCCs) critical for calcium ion influx in excitable cells. These channels are pivotal in regulating cellular processes such as action potential generation, excitation-contraction coupling in cardiac and smooth muscle, and neurotransmitter release in neurons. The CACNA1C-encoded protein consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6), with the S4 segment acting as a voltage sensor. Alternative splicing generates multiple isoforms with tissue-specific functions, particularly in the heart, brain, and endocrine systems.
Recombinant CACNA1C protein is engineered to study the structure, function, and dysregulation of CaV1.2 channels. It is commonly expressed in heterologous systems (e.g., HEK293 cells) alongside auxiliary subunits (β, α2δ) to recapitulate native channel properties. Researchers use this tool to investigate channel kinetics, pharmacology, and interactions with drugs targeting cardiovascular diseases (e.g., hypertension, arrhythmias) and neurological disorders. Mutations in CACNA1C are linked to Timothy syndrome, Brugada syndrome, and psychiatric conditions, making the recombinant protein invaluable for mechanistic studies and drug discovery. Its production typically involves affinity purification tags (e.g., GFP, His-tags) to facilitate isolation and functional validation via patch-clamp electrophysiology or fluorescence-based assays.
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