| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLRa2 |
| Uniprot No | P23416 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452aa |
| 氨基酸序列 | MNRQLVNILTALFAFFLETNHFRTAFCKDHDSRSGKQPSQTLSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQQWNDSRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRISKNGKVLYSIRLTLTLSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLSDGPVQVAEGLTLPQFILKEEKELGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAAVNFVSRQHKEFLRLRRRQKRQNKEEDVTRESRFNFSGYGMGHCLQVKDGTAVKATPANPLPQPPKDGDAIKKKFVDRAKRIDTISRAAFPLAFLIFNIFYWITYKIIRHEDVHKK |
| 预测分子量 | 52,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLRa2(Grapevine leafroll-associated virus 2)重组蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
1. **文献名称**：*Expression and Purification of Recombinant GLRa2 Coat Protein for Antibody Production*
**作者**：Smith A, et al.
**摘要**：本研究通过原核表达系统成功表达并纯化了GLRa2的外壳蛋白(CP)重组蛋白，并以此为抗原制备了高特异性的多克隆抗体。该抗体在ELISA和Western blot中表现出对天然病毒粒子的高效检测能力，为葡萄卷叶病诊断提供了新工具。
2. **文献名称**：*Development of a Serological Assay Based on GLRa2 Recombinant Protein for Virus Detection*
**作者**：Zhang L, et al.
**摘要**：作者利用昆虫细胞表达系统制备了GLRa2重组CP蛋白，开发了一种基于间接ELISA的检测方法。该方法可特异性识别不同地域的GLRa2分离株，灵敏度显著高于传统PCR技术，适用于大规模田间样本筛查。
3. **文献名称**：*Functional Analysis of GLRa2 Movement Protein Using Recombinant Protein Technology*
**作者**：García-Ruiz H, et al.
**摘要**：通过构建GLRa2运动蛋白(MP)的重组表达载体，研究发现该蛋白在植物细胞间转运中起关键作用。重组MP与宿主蛋白的互作实验揭示了病毒在葡萄韧皮部扩散的分子机制。
4. **文献名称**：*Comparative Study of GLRa2 and GLRa3 Recombinant Proteins in Cross-Protection*
**作者**：Wang Y, et al.
**摘要**：比较了GLRa2和GLRa3重组外壳蛋白的抗原性差异，发现两者存在部分交叉反应表位。基于重组蛋白的交叉保护实验表明，GLRa2可能通过诱导广谱抗性降低混合感染发生率。
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**注**：以上为模拟文献，实际研究中建议通过Web of Science、PubMed或Google Scholar以关键词“GLRa2 recombinant protein”或“Grapevine leafroll-associated virus 2”检索最新论文。真实研究多发表于*Plant Pathology*、*Archives of Virology*等期刊。
**Background of GLRaV-2 Recombinant Protein**
Grapevine leafroll-associated virus 2 (GLRaV-2), a member of the genus *Ampelovirus* in the family *Closteroviridae*, is a significant pathogen affecting grapevines worldwide. It is associated with grapevine leafroll disease (GLD), a complex viral syndrome that reduces yield, delays fruit ripening, and impairs wine quality. GLRaV-2 is transmitted by mealybugs and through grafting, posing challenges for vineyard management. Its genome consists of a single-stranded positive-sense RNA encoding proteins involved in replication, movement, and virion assembly.
Recombinant GLRaV-2 proteins are artificially expressed in heterologous systems (e.g., *E. coli* or insect cells*) to study viral pathogenesis, host interactions, and diagnostic applications. Key targets include the coat protein (CP), which is critical for viral particle formation, and the heat shock protein 70 homolog (Hsp70h), involved in cell-to-cell movement. These proteins are often purified for antibody production, enabling serological detection of the virus in infected plants.
The development of GLRaV-2 recombinant proteins has advanced research into viral protein functions and immune responses. For example, CP-based ELISA or lateral flow assays are used for rapid field diagnostics. Additionally, recombinant proteins aid in understanding viral suppression of host defense mechanisms, such as interference with RNA silencing. Efforts to engineer resistant grapevine varieties or cross-protective strategies also rely on insights from these proteins.
Overall, GLRaV-2 recombinant proteins serve as essential tools for diagnostics, functional virology studies, and the development of sustainable control measures against GLD, contributing to global viticulture health and productivity.
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