| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBA52 |
| Uniprot No | P62987 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-128aa |
| 氨基酸序列 | KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGII EPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK |
| 预测分子量 | 14,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBA52重组蛋白的3篇参考文献，列举格式符合要求：
1. **文献名称**: *"UBA52 regulates cell proliferation via the ubiquitin-proteasome system"*
**作者**: Zhang, Y. et al.
**摘要**: 研究报道了UBA52重组蛋白在HEK293细胞中的表达与纯化，并发现其通过调控泛素-蛋白酶体通路影响细胞周期蛋白降解，从而抑制癌细胞增殖。
2. **文献名称**: *"Structural insights into the UBA52-ribosomal fusion protein and its role in translation"*
**作者**: Lee, S. & Kim, J.H.
**摘要**: 利用X射线晶体学解析了重组UBA52蛋白的结构，揭示了其核糖体蛋白L40与泛素结构域的空间构象，探讨了其在翻译调控中的潜在作用。
3. **文献名称**: *"Expression and functional characterization of recombinant UBA52 in E. coli"*
**作者**: Müller, R. et al.
**摘要**: 研究优化了UBA52重组蛋白在大肠杆菌中的表达条件，开发了高效可溶性纯化方案，并验证了其体外泛素化活性及与蛋白酶体的相互作用。
(注：上述文献为模拟示例，实际引用需以真实文献为准。)
**Background of UBA52 Recombinant Protein**
UBA52. a ubiquitin-ribosomal fusion gene, encodes two distinct functional proteins: ubiquitin and ribosomal protein L40 (RPL40). Ubiquitin, a highly conserved 76-amino-acid protein, plays a central role in the ubiquitin-proteasome system (UPS), which regulates protein degradation, cellular homeostasis, and stress responses. By covalently attaching to substrate proteins, ubiquitin tags them for proteasomal degradation, influencing processes like cell cycle progression, DNA repair, and immune signaling.
The UBA52 gene produces a precursor protein that undergoes post-translational cleavage to release ubiquitin and RPL40. RPL40 is a component of the 60S ribosomal subunit, essential for protein synthesis. The fusion of ubiquitin and RPL40 in UBA52 highlights an evolutionary mechanism to ensure coordinated expression of ubiquitin and ribosomal proteins, critical for cell viability.
Recombinant UBA52 protein is engineered using expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce high-purity, functional ubiquitin or RPL40 for research. Its recombinant form enables studies on UPS dysregulation linked to diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), and autoimmune conditions. Researchers utilize UBA52-derived ubiquitin to investigate substrate recognition, deubiquitinating enzymes (DUBs), and drug development targeting the UPS.
Additionally, recombinant UBA52 aids in exploring ribosomal biogenesis defects and ribosomopathies. Its dual-function nature provides a unique model to study crosstalk between protein synthesis and degradation pathways. Advances in structural biology and proteomics have further leveraged recombinant UBA52 to map ubiquitin-protein interactions and develop therapeutic inhibitors.
In summary, UBA52 recombinant protein serves as a vital tool for dissecting ubiquitin-dependent pathways and ribosomal functions, offering insights into disease mechanisms and potential therapeutic strategies.
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