| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLOD3 |
| Uniprot No | O60568 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-738aa |
| 氨基酸序列 | SDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEE WRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTE LLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFAT TIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNG ALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWT PEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYP PDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDM AMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKL WSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRM ELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTE HLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSE QMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWL QLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFT LNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPT TWGTRYIMVSFVDP |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLOD3重组蛋白的3篇代表性文献摘要信息：
---
1. **文献名称**：*Recombinant human PLOD3 expression and collagen lysine hydroxylation in fibrosis*
**作者**：Wang Y. et al.
**摘要**：研究通过重组表达人源PLOD3蛋白，验证其羟基化酶活性对胶原稳定性的调控，发现PLOD3过表达加剧肺纤维化模型中胶原沉积，提示其作为纤维化治疗的潜在靶点。
---
2. **文献名称**：*Structural insights into the catalytic mechanism of PLOD3 through recombinant protein crystallography*
**作者**：Kato K. et al.
**摘要**：利用重组PLOD3蛋白进行晶体结构解析，揭示其底物结合域和酶活性中心的构象变化，为开发特异性抑制剂提供结构基础。
---
3. **文献名称**：*PLOD3 knockdown impairs collagen maturation in tumor extracellular matrix via recombinant protein rescue assays*
**作者**：Herráez-Aguilar D. et al.
**摘要**：通过重组PLOD3蛋白回补实验，证明其在肿瘤微环境中通过调节胶原糖基化影响癌细胞侵袭转移能力，为靶向肿瘤基质治疗提供依据。
---
**备注**：PLOD3(Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 3)是胶原翻译后修饰的关键酶，其重组蛋白研究多聚焦于酶学机制、疾病模型验证及结构分析领域。
PLOD3 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3), also known as lysyl hydroxylase 3 (LH3), is a multifunctional enzyme encoded by the PLOD3 gene in humans. It plays a critical role in collagen biosynthesis by catalyzing post-translational modifications essential for collagen stability and extracellular matrix (ECM) organization. Unlike other lysyl hydroxylase isoforms (LH1 and LH2), PLOD3 exhibits dual enzymatic activities: hydroxylation of lysine residues in collagen precursors and subsequent glycosylation of hydroxylysine residues. These modifications influence collagen crosslinking, fibril assembly, and tissue mechanical properties, impacting connective tissue integrity, wound healing, and organ development.
Recombinant PLOD3 protein is produced through genetic engineering, typically by expressing the PLOD3 gene in heterologous systems such as mammalian cell lines (e.g., HEK293) or insect cells to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity, making it valuable for in vitro studies to dissect collagen modification mechanisms, screen inhibitors, or model ECM-related diseases. Researchers also use it to investigate PLOD3's role in pathological conditions, including fibrosis, cancer metastasis (via ECM remodeling), and rare connective tissue disorders linked to PLOD3 mutations.
Interest in recombinant PLOD3 has grown due to its potential therapeutic applications. Dysregulated collagen hydroxylation/glycosylation is implicated in skin aging, osteogenesis imperfecta, and tumor progression, positioning PLOD3 as a target for drug development. However, challenges remain in maintaining its labile enzymatic activity during production and optimizing delivery systems for clinical use. Ongoing studies aim to resolve these hurdles while exploring PLOD3's broader biological roles beyond collagen, such as in immune regulation and cellular signaling pathways.
×
