| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADRb3 |
| Uniprot No | P13945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MAPWPHENSSLAPWPDLPTLAPNTANTSGLPGVPWEAALAGALLALAVLATVGGNLLVIVAIAWTPRLQTMTNVFVTSLAAADLVMGLLVVPPAATLALTGHWPLGATGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPLRYGALVTKRCARTAVVLVWVVSAAVSFAPIMSQWWRVGADAEAQRCHSNPRCCAFASNMPYVLLSSSVSFYLPLLVMLFVYARVFVVATRQLRLLRGELGRFPPEESPPAPSRSLAPAPVGTCAPPEGVPACGRRPARLLPLREHRALCTLGLIMGTFTLCWLPFFLANVLRALGGPSLVPGPAFLALNWLGYANSAFNPLIYCRSPDFRSAFRRLLCRCGRRLPPEPCAAARPALFPSGVPAARSSPAQPRLCQRLDGASWGVS |
| 预测分子量 | 49.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADRb3(β3-肾上腺素能受体)重组蛋白的3篇代表性文献示例，涵盖表达、功能及结构研究：
1. **标题**：*"Heterologous Expression and Functional Characterization of Human β3-Adrenergic Receptor in Escherichia coli"*
**作者**：Smith J, Doe R, Lee T.
**摘要**：本研究成功在*E. coli*中表达并纯化具有活性的ADRB3重组蛋白，通过优化跨膜结构域的截断设计和膜蛋白复性策略，证实其可与激动剂特异性结合，为抗肥胖药物的体外筛选提供了工具。
2. **标题**：*"Structural Insights into β3-Adrenergic Receptor Activation via Cryo-EM Analysis of Recombinant Protein"*
**作者**：Zhang Y, Wang H, Liu X.
**摘要**：利用昆虫细胞表达系统制备ADRB3重组蛋白，结合冷冻电镜技术解析其与激动剂复合物的三维结构，揭示了受体激活的关键构象变化，为靶向ADRB3的药物设计提供结构基础。
3. **标题**：*"β3-Adrenoceptor Recombinant Protein Modulates Lipolysis and Thermogenesis in Adipocytes"*
**作者**：Tanaka K, Sato M, Nakamura S.
**摘要**：通过哺乳动物细胞表达ADRB3重组蛋白，验证其在脂肪细胞中促进cAMP信号通路、脂解及产热的功能，证实其在代谢性疾病治疗中的潜在作用。
4. **标题**：*"High-Throughput Screening Platform for β3-AR Ligands Using Fluorescent-Labeled Recombinant Receptor"*
**作者**：Patel A, Johnson L.
**摘要**：开发基于荧光标记ADRB3重组蛋白的高通量药物筛选系统，成功鉴定多个新型激动剂/拮抗剂，加速抗糖尿病和肥胖症的先导化合物发现。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science检索真实文献(关键词：ADRB3 recombinant protein, heterologous expression, β3-adrenergic receptor structure)。
**Background of ADRb3 Recombinant Protein**
The β3-adrenergic receptor (ADRB3) is a member of the G protein-coupled receptor (GPCR) family, primarily involved in regulating metabolic processes such as lipolysis and thermogenesis. It is predominantly expressed in adipose tissue and plays a key role in activating signaling pathways that enhance energy expenditure. ADRB3 has garnered significant interest in metabolic research due to its potential implications in obesity, diabetes, and metabolic syndrome.
Recombinant ADRB3 protein is engineered in vitro using expression systems like *E. coli* or mammalian cell lines to study its structure, function, and interactions. This genetically modified protein retains the native receptor's structural features, including seven transmembrane domains, extracellular loops, and ligand-binding sites, enabling researchers to investigate its activation mechanisms and downstream signaling.
ADRB3’s therapeutic relevance stems from its ability to stimulate brown adipose tissue (BAT) activity and promote white adipose tissue (WAT) browning, processes linked to increased calorie burning. However, developing ADRB3-targeted drugs has been challenging due to the receptor’s conformational dynamics and species-specific ligand selectivity. Recombinant ADRB3 proteins facilitate high-throughput screening of agonists or antagonists, aiding drug discovery efforts for metabolic disorders.
Additionally, studies using recombinant ADRB3 have elucidated polymorphisms, such as the Trp64Arg variant, associated with reduced receptor activity and metabolic dysfunction. These insights highlight its role in personalized medicine approaches. Despite challenges in stabilizing the protein for structural studies, advancements in cryo-EM and computational modeling have improved understanding of ADRB3’s activation states.
Overall, recombinant ADRB3 serves as a critical tool for deciphering metabolic regulation mechanisms and accelerating therapies for obesity-related conditions.
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