| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RARb |
| Uniprot No | P10826 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455aa |
| 氨基酸序列 | MTTSGHACPV PAVNGHMTHY PATPYPLLFP PVIGGLSLPP LHGLHGHPPP SGCSTPSPAT IETQSTSSEE LVPSPPSPLP PPRVYKPCFV CQDKSSGYHY GVSACEGCKG FFRRSIQKNM IYTCHRDKNC VINKVTRNRC QYCRLQKCFE VGMSKESVRN DRNKKKKETS KQECTESYEM TAELDDLTEK IRKAHQETFP SLCQLGKYTT NSSADHRVRL DLGLWDKFSE LATKCIIKIV EFAKRLPGFT GLTIADQITL LKAACLDILI LRICTRYTPE QDTMTFSDGL TLNRTQMHNA GFGPLTDLVF TFANQLLPLE MDDTETGLLS AICLICGDRQ DLEEPTKVDK LQEPLLEALK IYIRKRRPSK PHMFPKILMK ITDLRSISAK GAERVITLKM EIPGSMPPLI QEMLENSEGH EPLTPSSSGN TAEHSPSISP SSVENSGVSQ SPLVQ |
| 预测分子量 | 50,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与RARβ重组蛋白相关的文献示例(信息基于公开研究整理)：
1. **《Recombinant human retinoic acid receptor beta: production, purification, and structural characterization》**
- **作者**：Smith J, et al.
- **摘要**：报道了人源RARβ重组蛋白在大肠杆菌中的高效表达和纯化方法，并通过质谱和圆二色谱验证其结构完整性，为后续功能研究提供基础。
2. **《Functional analysis of RARβ isoforms in cancer cell differentiation using recombinant proteins》**
- **作者**：Li X, Wang Y.
- **摘要**：利用哺乳动物细胞系统表达RARβ1和RARβ2重组蛋白，证明RARβ2通过调控靶基因转录显著抑制乳腺癌细胞增殖并诱导分化。
3. **《Crystal structure of the ligand-binding domain of RARβ bound to all-trans retinoic acid》**
- **作者**：Rochel N, et al.
- **摘要**：解析了RARβ配体结合域与全反式维甲酸复合物的晶体结构，揭示了其特异性结合的分子机制，为设计靶向药物提供依据。
4. **《Recombinant RARβ enhances the antitumor effect of cisplatin in lung adenocarcinoma via suppressing Wnt/β-catenin pathway》**
- **作者**：Chen L, et al.
- **摘要**：研究发现重组RARβ蛋白通过抑制Wnt通路增强肺癌细胞对顺铂的敏感性，提示其作为辅助治疗的潜在价值。
注：以上为模拟文献案例，实际引用时需核实具体论文信息。
**Background of RARβ Recombinant Protein**
The retinoic acid receptor beta (RARβ), a member of the nuclear receptor superfamily, plays a critical role in mediating the biological effects of retinoic acid (RA), a metabolite of vitamin A. As a ligand-activated transcription factor, RARβ regulates gene expression by binding to RA response elements (RAREs) in target gene promoters, often forming heterodimers with retinoid X receptors (RXRs). This receptor is involved in cellular processes such as differentiation, proliferation, apoptosis, and embryonic development.
RARβ exists in multiple isoforms (e.g., RARβ1. β2. β4) due to alternative splicing, with RARβ2 being the most studied due to its tumor-suppressive properties. Dysregulation of RARβ expression is linked to various cancers, including lung, breast, and head and neck cancers, where its downregulation is associated with poor prognosis and resistance to retinoid therapy.
Recombinant RARβ protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. It enables studies on RA signaling mechanisms, structural analysis of receptor-ligand interactions, and screening of synthetic retinoids for therapeutic applications. In cancer research, recombinant RARβ is used to explore its role in restoring retinoid sensitivity and suppressing oncogenic pathways. Additionally, it serves as a tool for developing targeted therapies and biomarkers in precision medicine.
Overall, RARβ recombinant protein is vital for dissecting retinoid biology and advancing translational research in oncology and developmental disorders.
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