| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTB |
| Uniprot No | P60709 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF |
| 预测分子量 | 44.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTB重组蛋白的模拟参考文献示例(实际引用时请核实文献真实性)：
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1. **文献名称**：*"High-yield expression and purification of human β-actin in Escherichia coli for structural studies"*
**作者**：Zhang et al.
**摘要**：研究报道了人源ACTB基因在大肠杆菌中的高效重组表达及纯化方法，通过优化密码子和诱导条件获得高纯度β-actin蛋白，用于X射线晶体学分析。
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2. **文献名称**：*"Functional characterization of recombinant β-actin in cytoskeletal dynamics"*
**作者**：Thompson & Pollard
**摘要**：利用昆虫细胞系统表达重组β-actin，验证其与天然蛋白在细胞骨架组装和肌动蛋白丝形成中的功能等效性，为体外细胞迁移研究提供工具。
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3. **文献名称**：*"A novel tag-free strategy for purification of bioactive ACTB recombinant protein"*
**作者**：Kim et al.
**摘要**：开发无标签的ACTB重组蛋白纯化技术，避免标签对蛋白功能干扰，证明其在体外结合实验(如Cofilin相互作用)中的活性。
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4. **文献名称**：*"Recombinant β-actin as a universal loading control in Western blot analysis"*
**作者**：Miller & Garcia-Sastre
**摘要**：评估重组β-actin作为内参蛋白的稳定性，比较其与天然β-actin在多种细胞系Western blot中的一致性，提出标准化实验方案。
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**提示**：建议通过PubMed、Google Scholar等平台以关键词“recombinant ACTB”、“beta-actin expression”检索最新文献，并核对作者及期刊信息。
**Background of ACTB Recombinant Protein**
ACTB, encoding β-actin, is a highly conserved cytoskeletal protein essential for maintaining cell structure, motility, and intracellular transport. As a core component of microfilaments, β-actin polymerizes into filaments that interact with myosin to enable mechanical functions, including cell division, migration, and signaling. Its ubiquitous expression across eukaryotic cells makes it a widely used loading control in molecular biology assays, such as Western blotting and qPCR.
The production of recombinant ACTB protein leverages genetic engineering to express β-actin in heterologous systems like *E. coli*, insect cells, or mammalian cell lines. Recombinant technology enables large-scale, high-purity yields of the protein while minimizing contamination from endogenous cellular factors. Tags (e.g., His, GST, or FLAG) are often fused to the protein to facilitate purification and detection.
ACTB recombinant proteins are critical tools for studying cytoskeletal dynamics, cell mechanics, and diseases linked to actin dysregulation, such as cancer metastasis, neurodegenerative disorders, and cardiovascular conditions. They are also employed in drug screening to identify compounds modulating actin polymerization or cytoskeletal stability. Additionally, these proteins aid in antibody validation and structural studies, providing insights into actin-binding partner interactions.
Despite conservation, post-translational modifications (e.g., acetylation, phosphorylation) vary depending on the expression host, potentially influencing experimental outcomes. Researchers must select recombinant ACTB tailored to their model system to ensure biological relevance. Overall, ACTB recombinant proteins bridge fundamental research and therapeutic development, underscoring their versatility in biomedical science.
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