| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKa |
| Uniprot No | P23743 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-735aa |
| 氨基酸序列 | MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGY EGFQQFLKIYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVS CYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLD WDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTL KDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKA LPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHC VWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKT SQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYI LNPRQVFNLLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANL PVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRW SVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNS RMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVL NIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLS DKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGE PWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKa重组蛋白的3篇参考文献示例(注：部分信息为模拟内容，实际文献需通过学术数据库检索确认)：
1. **"Recombinant expression and functional characterization of human diacylglycerol kinase alpha"**
*作者：Smith J, et al.*
**摘要**：本研究成功在大肠杆菌中表达并纯化了人源DGKa重组蛋白，通过体外酶活实验证实其催化二酰基甘油(DAG)转化为磷脂酸的活性，并发现其活性受钙离子浓度调节。
2. **"Structural insights into DGKα lipid kinase domain by X-ray crystallography"**
*作者：Tanaka R, et al.*
**摘要**：利用昆虫细胞系统表达重组DGKa激酶结构域，通过X射线晶体学解析其三维结构，揭示了底物结合口袋的关键氨基酸残基，为设计靶向抑制剂奠定基础。
3. **"DGKα recombinant protein suppresses tumor growth via modulating EGFR signaling in vitro and in vivo"**
*作者：Chen L, et al.*
**摘要**：通过哺乳动物细胞表达体系制备功能性DGKa重组蛋白，实验证明其过表达可抑制癌细胞增殖，机制可能与下调EGFR/Akt信号通路及降低DAG水平相关。
建议通过PubMed或Google Scholar以关键词 **"DGKα recombinant protein"** 或 **"diacylglycerol kinase alpha expression"** 检索最新文献获取准确信息。
**Background of DGKα Recombinant Protein**
Diacylglycerol kinase alpha (DGKα) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA), regulating the balance of these two critical lipid second messengers. DGKα belongs to the type I DGK family, characterized by calcium-binding EF-hand motifs and a pleckstrin homology domain, which mediate its cellular localization and activity. It plays pivotal roles in signaling pathways linked to cell proliferation, apoptosis, and immune responses, particularly in T-cell receptor signaling. Dysregulation of DGKα has been implicated in immune disorders, cancer, and neurological diseases, making it a therapeutic target of interest.
Recombinant DGKα protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper post-translational modifications and functional activity. Its production enables in vitro studies to dissect enzymatic mechanisms, screen inhibitors, or explore interactions with signaling partners. Researchers utilize DGKα recombinant protein to investigate its role in dampening DAG-mediated signals (e.g., Ras/ERK activation) while promoting PA-dependent pathways (e.g., mTOR signaling).
Recent studies highlight DGKα's dual role in cancer: suppressing oncogenic DAG signaling in some contexts while supporting tumor survival via PA production in others. In immunology, DGKα deficiency enhances T-cell activation, suggesting potential in adoptive immunotherapy. The recombinant protein also aids structural studies to design isoform-specific inhibitors, addressing challenges posed by functional redundancy among DGK isoforms. Overall, DGKα recombinant protein is a vital tool for unraveling lipid signaling complexity and developing targeted therapies.
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