| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPOR |
| Uniprot No | P40238 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-353aa |
| 氨基酸序列 | QVSSQDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPR EKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRT QRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRY GPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDG PKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGS WSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRAR CCPRDRYPIWENCEEEEKTNPGLQTPQFSRCH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPOR(促血小板生成素受体)重组蛋白的3篇代表性文献，涵盖结构、功能及药物开发研究：
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1. **文献名称**：*Molecular cloning and characterization of MPL, the human homolog of the v-mpl oncogene: a member of the hematopoietic cytokine receptor family*
**作者**：Vigon, I., et al.
**期刊/年份**：*Proc Natl Acad Sci USA*, 1992
**摘要**：该研究首次克隆并鉴定了人源TPOR(MPL)基因，证实其属于造血细胞因子受体超家族，并揭示了其在巨核细胞分化与血小板生成中的关键作用，为后续重组蛋白研究奠定基础。
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2. **文献名称**：*Structural basis for activation of the thrombopoietin receptor*
**作者**：Staerk, J., et al.
**期刊/年份**：*Nature*, 2015
**摘要**：通过冷冻电镜技术解析TPO与TPOR胞外域的复合物结构，揭示了受体二聚化及激活机制，为设计靶向TPOR的重组蛋白药物提供结构生物学依据。
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3. **文献名称**：*Eltrombopag, a small molecule agonist of the thrombopoietin receptor, acts through a mechanism distinct from recombinant TPO*
**作者**：Erickson-Miller, C.L., et al.
**期刊/年份**：*Blood*, 2007
**摘要**：研究对比了小分子激动剂艾曲波帕与重组TPO的作用机制，证明其通过结合TPOR跨膜区激活下游信号通路，突显重组TPOR蛋白在药物筛选中的工具价值。
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如需扩展，可补充关于TPOR-JAK2信号通路的研究文献(如Broudy, V.C., *Blood*, 1997)。以上文献均聚焦TPOR重组蛋白的分子机制与应用，涵盖基础研究至转化医学领域。
**Background of TPOR Recombinant Protein**
Thrombopoietin receptor (TPOR), also known as myeloproliferative leukemia protein (MPL), is a critical cytokine receptor belonging to the type I hematopoietic superfamily. It plays a pivotal role in regulating megakaryopoiesis and platelet production by binding to its ligand, thrombopoietin (TPO). Structurally, TPOR is a transmembrane protein comprising an extracellular TPO-binding domain, a single transmembrane helix, and an intracellular domain that activates downstream signaling pathways, primarily JAK-STAT, upon ligand-induced dimerization. Dysregulation of TPOR signaling is linked to hematologic disorders, including thrombocytopenia, myelofibrosis, and certain leukemias.
Recombinant TPOR proteins are engineered using DNA technology to express specific functional domains (e.g., extracellular regions) in host systems like *E. coli* or mammalian cells. These proteins retain the receptor’s ligand-binding capacity and are essential tools for studying TPO-TPOR interactions, screening therapeutic agents (e.g., TPO mimetics or antagonists), and developing diagnostic assays. For instance, drugs like romiplostim and eltrombopag, used to treat immune thrombocytopenia, function by activating TPOR signaling. Conversely, recombinant TPOR fragments can help identify inhibitors for conditions involving aberrant receptor activation.
The development of TPOR recombinant proteins has advanced both basic research and clinical applications, offering insights into receptor mechanics and enabling targeted therapies. Their stability, scalability, and specificity make them valuable in drug discovery and hematology research, bridging molecular understanding with therapeutic innovation.
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