| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSP |
| Uniprot No | P26927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MGWLPLLLLLTQCLGVPGQRSPLNDFQVLRGTELQHLLHAVVPGPWQEDVADAEECAGRCGPLMDCRAFHYNVSSHGCQLLPWTQHSPHTRLRRSGRCDLFQKKDYVRTCIMNNGVGYRGTMATTVGGLPCQAWSHKFPNDHKYTPTLRNGLEENFCRNPDGDPGGPWCYTTDPAVRFQSCGIKSCREAACVWCNGEEYRGAVDRTESGRECQRWDLQHPHQHPFEPGKFLDQGLDDNYCRNPDGSERPWCYTTDPQIEREFCDLPRCGSEAQPRQEATTVSCFRGKGEGYRGTANTTTAGVPCQRWDAQIPHQHRFTPEKYACKDLRENFCRNPDGSEAPWCFTLRPGMRAAFCYQIRRCTDDVRPQDCYHGAGEQYRGTVSKTRKGVQCQRWSAETPHKPQFTFTSEPHAQLEENFCRNPDGDSHGPWCYTMDPRTPFDYCALRRCADDQPPSILDPPDQVQFEKCGKRVDRLDQRRSKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWILTARQCFSSCHMPLTGYEVWLGTLFQNPQHGEPSLQRVPVAKMVCGPSGSQLVLLKLERSVTLNQRVALICLPPEWYVVPPGTKCEIAGWGETKGTGNDTVLNVALLNVISNQECNIKHRGRVRESEMCTEGLLAPVGACEGDYGGPLACFTHNCWVLEGIIIPNRVCARSRWPAVFTRVSVFVDWIHKVMRLG |
| 预测分子量 | 80,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MSP(Membrane Scaffold Protein)重组蛋白的经典文献摘要概括：
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1. **文献名称**：*Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins*
**作者**：Bayburt, T. H., Grinkova, Y. V., & Sligar, S. G.
**摘要**：该研究首次提出利用重组表达的MSP蛋白(如MSP1)与磷脂结合，自组装形成纳米脂质盘(Nanodiscs)，用于稳定膜蛋白(如细胞色素P450)。MSP通过大肠杆菌表达纯化，并验证了其与脂质的自组装机制，为膜蛋白体外研究提供了新工具。
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2. **文献名称**：*Directed Self-Assembly of Monodisperse Phospholipid Bilayer Nanodiscs with Controlled Size*
**作者**：Denisov, I. G., & Sligar, S. G.
**摘要**：作者通过工程化改造MSP蛋白(如MSP1E3D1)，调控纳米盘的直径(8-16 nm)，实现了对膜蛋白嵌入尺寸的精确控制。研究展示了MSP重组蛋白的灵活性和可扩展性，为不同膜蛋白的结构与功能研究提供了适配方案。
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3. **文献名称**：*Engineering Membrane Scaffold Proteins to Drive Soluble Nanodisc Formation*
**作者**：Ritchie, T. K., et al.
**摘要**：该文献报道了一种新型MSP突变体(如MSP2N2)，通过优化重组蛋白的疏水相互作用区域，显著提高了纳米盘的溶解性和稳定性。实验证明该突变体适用于G蛋白偶联受体(GPCR)等难溶性膜蛋白的功能分析。
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这些文献聚焦于MSP重组蛋白的设计、优化及其在膜蛋白研究中的关键作用，涵盖基础构建到应用拓展，被广泛引用于结构生物学和生物技术领域。
Membrane Scaffold Protein (MSP) recombinant proteins are engineered polypeptides central to the formation of nanodiscs, a groundbreaking tool in membrane protein research. Derived from apolipoproteins, particularly human apolipoprotein A-I, MSPs are amphipathic alpha-helical molecules that self-assemble with lipids to create stable, soluble discoidal lipid bilayers. These nanodiscs mimic native cell membrane environments, enabling the isolation and study of membrane proteins—such as receptors, transporters, and enzymes—in a functional, water-soluble state.
The development of MSPs, pioneered by Stephen G. Sligar’s group in the early 2000s, addressed longstanding challenges in handling membrane proteins, which are notoriously difficult to stabilize outside lipid bilayers. Recombinant DNA technology allows customizable MSP production, with variants like MSP1D1 and MSP1E3D1 offering tunable nanodisc sizes (5–17 nm) by altering the number of helical repeats. This flexibility accommodates diverse membrane proteins and complexes.
MSP-based nanodiscs have revolutionized structural biology (e.g., cryo-EM and X-ray crystallography), drug discovery, and functional studies by preserving protein conformation and activity. They also facilitate high-throughput screening of membrane protein-targeted therapeutics and enable investigations into lipid-protein interactions. Recent advances include hybrid nanodisc systems and conjugation strategies for biotechnological applications like biosensors or targeted drug delivery.
Despite their utility, challenges persist in optimizing homogeneity and scalability. Ongoing research focuses on expanding MSP versatility, such as pH-responsive designs or modified lipid compositions, to better replicate complex cellular membranes. MSP technology continues to bridge the gap between in vitro and in vivo studies, underpinning innovations across biochemistry and medicine.
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