| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Caspr |
| Uniprot No | P78357 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-356aa |
| 氨基酸序列 | DEELVGPLYARSLGASSYYSLLTAPRFARLHGISGWSPRIGDPNPWLQIDLMKKHRIRAVATQGSFNSWDWVTRYMLLYGDRVDSWTPFYQRGHNSTFFGNVNESAVVRHDLHFHFTARYIRIVPLAWNPRGKIGLRLGLYGCPYKADILYFDGDDAISYRFPRGVSRSLWDVFAFSFKTEEKDGLLLHAEGAQGDYVTLELEGAHLLLHMSLGSSPIQPRPGHTTVSAGGVLNDQHWHYVRVDRFGRDVNFTLDGYVQRFILNGDFERLNLDTEMFIGGLVGAARKNLAYRHNFRGCIENVIFNRVNIADLAVRRHSRITFEGKVAFRCL |
| 预测分子量 | 53.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Caspr(CNTNAP1)重组蛋白的参考文献示例，涵盖其结构、功能及疾病关联研究：
---
1. **标题**: *Structural characterization of the extracellular domain of Caspr/CNTNAP1 using recombinant expression*
**作者**: Smith A, et al.
**摘要**: 本研究通过哺乳动物细胞系统成功表达了Caspr的胞外结构域重组蛋白，利用X射线晶体学解析其三维结构，揭示了Caspr与Contactin蛋白相互作用的分子基础，为神经髓鞘形成的分子机制提供结构依据。
---
2. **标题**: *Autoantibodies against Caspr in autoimmune neuropathies: Role of recombinant Caspr in diagnostic assays*
**作者**: Lee B, et al.
**摘要**: 研究团队通过昆虫细胞表达系统制备了重组Caspr蛋白，并开发基于该蛋白的ELISA检测法，发现部分自身免疫性神经疾病患者血清中存在Caspr抗体，提示其作为潜在生物标志物的价值。
---
3. **标题**: *Functional analysis of Caspr mutations in neurodevelopmental disorders via recombinant protein modeling*
**作者**: Garcia-Ramos C, et al.
**摘要**: 通过大肠杆菌表达系统获得Caspr关键结构域的重组蛋白，结合CRISPR细胞模型，证明特定突变导致Caspr与细胞骨架蛋白结合能力下降，可能与髓鞘发育异常相关。
---
4. **标题**: *Recombinant Caspr-mediated neuronal adhesion assays in vitro*
**作者**: Tanaka K, et al.
**摘要**: 利用HEK293细胞表达全长Caspr重组蛋白，证实其通过与Contactin-1的相互作用促进神经元-胶质细胞粘附，为研究轴突-胶质相互作用提供了体外实验工具。
---
注：上述文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文章。
Caspr (Contactin-associated protein), also known as CNTNAP1. is a transmembrane protein belonging to the neurexin superfamily, primarily recognized for its critical role in nervous system function. It was first identified through its interaction with Contactin-1. a cell adhesion molecule, forming a cis-complex essential for the organization of myelinated axons. Structurally, Caspr contains extracellular domains with laminin G and epidermal growth factor (EGF)-like motifs, a transmembrane region, and a cytoplasmic tail that interacts with cytoskeletal proteins. This architecture enables its participation in cell-cell adhesion, axon-guidance, and the stabilization of ion channel clusters at specialized domains of myelinated fibers.
In the nervous system, Caspr is predominantly localized to the paranodal regions of myelinated axons, where it contributes to the assembly of the axon-glia junction. This junction is vital for maintaining the integrity of myelin sheaths and ensuring rapid saltatory conduction of nerve impulses. Studies have linked Caspr dysfunction to neurological disorders, including autoimmune neuropathies like multiple sclerosis, where disrupted paranodal structures impair nerve signaling. Additionally, mutations in the CNTNAP1 gene are associated with severe neurodevelopmental conditions, such as congenital hypomyelinating neuropathy, highlighting its importance in early neural development.
Recombinant Caspr proteins are engineered using expression systems (e.g., mammalian or insect cells) to preserve post-translational modifications critical for function. These proteins serve as tools for studying Caspr-mediated molecular interactions, screening therapeutic agents for demyelinating diseases, and developing diagnostic assays for autoimmune disorders targeting paranodal proteins. Recent research also explores Caspr's potential roles beyond myelination, including synaptic plasticity and neuroimmune crosstalk, broadening its relevance in both basic and clinical neuroscience.
×
