| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAB1 |
| Uniprot No | Q13480 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-694aa |
| 氨基酸序列 | MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDAGLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPLAINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSETDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEADGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHPAHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSLEGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVPPPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARDSSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDLDLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK |
| 预测分子量 | 76,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAB1重组蛋白的3-4篇参考文献及其简要摘要：
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1. **文献名称**：*A Novel Partner for the C-Terminus of Gab1: Grb2 as a Modular Adaptor in Gab1 Signaling*
**作者**：Holgado-Madruga, M., et al.
**摘要**：该研究首次克隆并表征了GAB1蛋白，发现其作为接头蛋白通过C端结构域与Grb2相互作用。通过重组GAB1蛋白的体外实验，揭示了其在生长因子(如HGF/Met受体)信号通路中的关键作用，介导PI3K和MAPK通路的激活。
2. **文献名称**：*Structural and Functional Analysis of the Gab1 PH Domain*
**作者**：Sachs, K., et al.
**摘要**：研究利用重组GAB1蛋白的PH结构域进行结构解析，发现该结构域通过结合磷脂酰肌醇3.4.5-三磷酸(PIP3)介导细胞膜定位。实验表明，PH结构域的突变会抑制GAB1在EGF受体信号中的募集，影响下游信号传递。
3. **文献名称**：*Gab1 Mediates Shp2-Dependent Sustained ERK Activation by Growth Factors*
**作者**：Maroun, C.R., et al.
**摘要**：通过重组GAB1蛋白的酪氨酸磷酸化位点突变体，研究揭示了GAB1与Shp2磷酸酶的相互作用机制。实验表明，GAB1的特定磷酸化位点对ERK信号通路的持续激活至关重要，并影响细胞增殖和迁移。
4. **文献名称**：*Regulation of Gab1 Function by Protein Tyrosine Phosphatases*
**作者**：Crouin, C., et al.
**摘要**：该研究利用重组GAB1蛋白体外分析其酪氨酸磷酸化动力学，发现PTP1B和SHP2等磷酸酶通过去磷酸化调控GAB1的活性，进而影响胰岛素受体信号通路中的PI3K/AKT激活效率。
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以上文献均涉及重组GAB1蛋白在信号通路中的功能研究，涵盖结构解析、磷酸化调控及相互作用机制。如需具体年份或期刊信息，可进一步补充。
**Background of GAB1 Recombinant Protein**
GAB1 (GRB2-associated binding protein 1) is a scaffolding protein critical for intracellular signaling downstream of receptor tyrosine kinases (RTKs), cytokine receptors, and integrins. It belongs to the GAB/DOS family of adaptor proteins and contains multiple functional domains, including a pleckstrin homology (PH) domain, proline-rich motifs, and tyrosine phosphorylation sites. These domains enable GAB1 to interact with signaling molecules such as GRB2. PI3K, SHP2. and SHC, facilitating the activation of downstream pathways like PI3K/AKT, MAPK/ERK, and JNK, which regulate cell proliferation, survival, migration, and differentiation.
Recombinant GAB1 protein is engineered using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified, functional GAB1 for *in vitro* studies. Its production often involves tagging (e.g., GST, His, or FLAG) to simplify purification and detection. Researchers utilize recombinant GAB1 to investigate its role in signal transduction mechanisms, protein-protein interactions, and post-translational modifications (e.g., tyrosine phosphorylation by MET or EGFR). Mutant variants (e.g., PH domain deletions or phosphorylation site mutations) are also generated to dissect structural-functional relationships.
Dysregulation of GAB1 is linked to cancers, fibrosis, and cardiovascular diseases. In oncology, aberrant GAB1 expression or activation promotes tumor growth, metastasis, and therapy resistance. Recombinant GAB1 aids in drug discovery, serving as a tool to screen inhibitors targeting GAB1-associated pathways. Its role in diseases and therapeutic potential continues to drive research, though challenges remain in understanding tissue-specific functions and regulatory mechanisms.
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