| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DVL1 |
| Uniprot No | O14640 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-110aa |
| 氨基酸序列 | MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFK SMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHT DLPPPLERTG |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DVL1重组蛋白的3篇参考文献(示例为虚构，仅供参考格式)：
1. **文献名称**：*Structural insights into DVL1 recombinant protein and its role in Wnt signaling*
**作者**：Smith A, et al.
**摘要**：研究报道了重组人DVL1蛋白的晶体结构，解析了其与β-catenin的相互作用界面，揭示了其在Wnt/β-catenin信号通路中的构象调控机制。
2. **文献名称**：*Expression and purification of functional DVL1 recombinant protein in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，用于生产具有生物活性的重组DVL1蛋白，并通过体外实验验证其促进细胞迁移的功能。
3. **文献名称**：*DVL1 recombinant protein enhances planar cell polarity in mammalian cells*
**作者**：Johnson R, et al.
**摘要**：利用重组DVL1蛋白处理哺乳动物细胞，发现其显著增强平面细胞极性(PCP)通路活性，为发育生物学研究提供了工具蛋白。
(注：以上文献为示例，实际引用需根据具体研究内容检索PubMed或Google Scholar获取真实文献。)
**Background of DVL1 Recombinant Protein**
Dishevelled-1 (DVL1) is a key cytoplasmic phosphoprotein belonging to the Dishevelled (DVL) family, which includes three paralogs (DVL1. DVL2. DVL3) in mammals. These proteins are evolutionarily conserved and play central roles in Wnt signaling pathways, which regulate critical biological processes such as embryonic development, cell polarity, proliferation, and tissue homeostasis. DVL1 acts as a scaffold protein, mediating signal transduction in both the canonical Wnt/β-catenin pathway and non-canonical Wnt/planar cell polarity (PCP) pathways. Its dysfunction is implicated in developmental disorders, cancer, and other diseases.
Structurally, DVL1 contains three conserved domains: the DIX domain (essential for β-catenin-dependent signaling), the PDZ domain (mediates protein-protein interactions), and the DEP domain (critical for PCP signaling). Post-translational modifications, such as phosphorylation and ubiquitination, tightly regulate its activity and stability. Recombinant DVL1 proteins are engineered to study these molecular mechanisms, often expressed in *E. coli* or mammalian systems with tags (e.g., GST, His, or FLAG) for purification and detection.
Research applications of recombinant DVL1 include *in vitro* binding assays, structural studies, and functional analyses to dissect Wnt pathway dynamics. It is also used to investigate pathological contexts, such as cancer metastasis, where DVL1 overexpression correlates with enhanced Wnt signaling and tumor aggressiveness. Additionally, recombinant DVL1 aids in drug discovery, serving as a target for inhibitors aiming to modulate aberrant Wnt activity. Recent studies highlight its potential role in regenerative medicine, particularly in stem cell differentiation and tissue repair.
Overall, DVL1 recombinant protein is a vital tool for unraveling Wnt signaling complexity and developing therapeutic strategies for Wnt-related diseases.
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