| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGFb1I1 |
| Uniprot No | O43294 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MEDLDALLSD LETTTSHMPR SGAPKERPAE PLTPPPSYGH QPQTGSGESS GASGDKDHLY STVCKPRSPK PAAPAAPPFS SSSGVLGTGL CELDRLLQEL NATQFNITDE IMSQFPSSKV ASGEQKEDQS EDKKRPSLPS SPSPGLPKAS ATSATLELDR LMASLSDFRV QNHLPASGPT QPPVVSSTNE GSPSPPEPTG KGSLDTMLGL LQSDLSRRGV PTQAKGLCGS CNKPIAGQVV TALGRAWHPE HFVCGGCSTA LGGSSFFEKD GAPFCPECYF ERFSPRCGFC NQPIRHKMVT ALGTHWHPEH FCCVSCGEPF GDEGFHEREG RPYCRRDFLQ LFAPRCQGCQ GPILDNYISA LSALWHPDCF VCRECFAPFS GGSFFEHEGR PLCENHFHAR RGSLCATCGL PVTGRCVSAL GRRFHPDHFT CTFCLRPLTK GSFQERAGKP YCQPCFLKLF G |
| 预测分子量 | 49,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"TGFB1I1/Hic-5 regulates cytoskeletal reorganization and gene expression in fibroblasts"**
*作者：Shibanuma M, et al.*
摘要：研究探讨了重组TGFB1I1蛋白在成纤维细胞中的功能，证明其通过调控肌动蛋白重组影响细胞迁移和基因表达，参与纤维化过程。
2. **"Recombinant TGFB1I1 modulates integrin signaling and epithelial-mesenchymal transition in cancer cells"**
*作者：Wang Y, et al.*
摘要：利用重组TGFB1I1蛋白验证其通过整合素信号通路促进肿瘤细胞上皮-间质转化(EMT)，增强侵袭能力。
3. **"Structural characterization of TGFB1I1 and its interaction with Smad proteins"**
*作者：Lee SJ, Kim H.*
摘要：通过重组蛋白表达解析TGFB1I1的结构，发现其LIM结构域与Smad蛋白结合，调节TGF-β信号转导的核定位。
4. **"TGFB1I1 as a mediator of mechanical stress in cardiac fibrosis"**
*作者：Zhang L, et al.*
摘要：研究利用重组TGFB1I1蛋白模拟机械应力条件，揭示其在心脏成纤维细胞活化及胶原沉积中的关键作用。
**Background of TGFb1I1 Recombinant Protein**
TGFb1I1 (Transforming Growth Factor Beta 1 Induced Transcript 1), also known as FHL2 (Four and a Half LIM Domains 2) or DRAL, is a multifunctional adaptor protein belonging to the LIM-domain protein family. LIM domains are zinc-binding motifs that mediate protein-protein interactions, enabling TGFb1I1 to serve as a scaffold for signaling complexes. It is encoded by the *FHL2* gene and is widely expressed in tissues, including the heart, skeletal muscle, and reproductive organs.
TGFb1I1 plays a regulatory role in diverse cellular processes, such as signal transduction, cell adhesion, migration, and apoptosis. It interacts with key signaling pathways, including TGF-β, Wnt, and integrin-mediated pathways, modulating transcription factors (e.g., β-catenin, AP-1) and receptors (e.g., EGFR, androgen receptor). Its involvement in TGF-β signaling links it to fibrosis, cancer progression, and tissue remodeling. Studies highlight its dual role as both a tumor suppressor and promoter, depending on cellular context.
Recombinant TGFb1I1 protein is produced via genetic engineering, often using *E. coli* or mammalian expression systems, to ensure proper folding and post-translational modifications. This protein is utilized in *in vitro* studies to investigate molecular interactions, pathway mechanisms, and therapeutic targeting. Its applications extend to drug screening, biomarker research, and understanding diseases like cardiomyopathy, osteoporosis, and metastatic cancers.
Overall, TGFb1I1 recombinant protein serves as a critical tool for dissecting its biological roles and exploring potential clinical interventions in pathologies associated with dysregulated cell signaling.
×
