| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IkBz |
| Uniprot No | Q9BYH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718aa |
| 氨基酸序列 | MIVDKLLDDS RGGEGLRDAA GGCGLMTSPL NLSYFYGASP PAAAPGACDA SCSVLGPSAP GSPGSDSSDF SSASSVSSCG AVESRSRGGA RAERQPVEPH MGVGRQQRGP FQGVRVKNSV KELLLHIRSH KQKASGQAVD DFKTQGVNIE QFRELKNTVS YSGKRKGPDS LSDGPACKRP ALLHSQFLTP PQTPTPGESM EDVHLNEPKQ ESSADLLQNI INIKNECSPV SLNTVQVSWL NPVVVPQSSP AEQCQDFHGG QVFSPPQKCQ PFQVRGSQQM IDQASLYQYS PQNQHVEQQP HYTHKPTLEY SPFPIPPQSP AYEPNLFDGP ESQFCPNQSL VSLLGDQRES ENIANPMQTS SSVQQQNDAH LHSFSMMPSS ACEAMVGHEM ASDSSNTSLP FSNMGNPMNT TQLGKSLFQW QVEQEESKLA NISQDQFLSK DADGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NIGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVGSN QFVDLEATNY DGLTPLHCAV IAHNAVVHEL QRNQQPHSPE VQELLLKNKS LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNAKAYNGN TALHVAASLQ YRLTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE QIRRILKGKS IQQRAPPY |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **IκBζ(IkBz)重组蛋白** 的3篇参考文献示例(内容为模拟，仅供参考)：
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1. **文献名称**: *"IκBζ: A critical regulator of NF-κB transcriptional activity in innate immunity"*
**作者**: Hiroaki et al.
**摘要**: 该研究首次鉴定了IκBζ作为NF-κB信号通路的新型调控因子，发现其通过结合p50亚基抑制基础NF-κB活性，但在TLR激活后促进特定炎症基因(如IL-6)的表达。重组IκBζ蛋白被用于体外结合实验，证实其与NF-κB的相互作用。
2. **文献名称**: *"Recombinant IκBζ protein suppresses LPS-induced septic shock by modulating NF-κB dynamics"*
**作者**: Yamamoto et al.
**摘要**: 研究利用大肠杆菌表达系统纯化重组IκBζ蛋白，并在小鼠败血症模型中验证其治疗潜力。结果显示，重组IκBζ能选择性抑制促炎因子(如TNF-α)，但不影响抗凋亡基因，提示其作为抗炎药物的潜力。
3. **文献名称**: *"Structural basis of IκBζ function in dendritic cell maturation"*
**作者**: Sasaki et al.
**摘要**: 通过X射线晶体学解析重组IκBζ蛋白的C端结构域，揭示其与DNA结合域的相互作用机制。实验表明，IκBζ缺失突变体重组体无法促进树突状细胞成熟，证实其结构对免疫细胞分化的必要性。
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*注：以上为模拟文献，实际研究中建议通过PubMed或Google Scholar以关键词“IκBζ recombinant protein”“NFKBIAZ”检索最新论文。*
IκBζ (NFKBIZ), a member of the IκB family, is a key regulatory protein in the NF-κB signaling pathway. Unlike classical IκB proteins (e.g., IκBα) that sequester NF-κB in the cytoplasm, IκBζ is synthesized upon stimulation (e.g., via TLRs or IL-1R) and primarily modulates NF-κB activity in the nucleus. It contains ankyrin repeats for protein interactions and a nuclear localization signal. IκBζ acts as a coactivator or corepressor, fine-tuning NF-κB target gene expression, particularly in inflammatory responses (e.g., regulating IL-6. IL-12). Its dual role—enhancing anti-inflammatory IL-10 while suppressing pro-inflammatory cytokines—highlights its complex regulatory function.
Recombinant IκBζ proteins are engineered using systems like E. coli or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. These proteins enable studies on IκBζ’s structure, interactions (e.g., with p50 or RelA subunits), and mechanisms in diseases like rheumatoid arthritis, psoriasis, and cancer. Dysregulation of IκBζ is linked to chronic inflammation and immune disorders, making it a potential therapeutic target. Research using recombinant IκBζ aids in developing inhibitors or modulators to restore NF-κB balance, offering insights into precision treatments for inflammation-driven pathologies.
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