| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGaD |
| Uniprot No | Q13349 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1161aa |
| 氨基酸序列 | MTFGTVLLLSVLASYHGFNLDVEEPTIFQEDAGGFGQSVVQFGGSRLVVGAPLEVVAANQTGRLYDCAAATGMCQPIPLHIRPEAVNMSLGLTLAASTNGSRLLACGPTLHRVCGENSYSKGSCLLLGSRWEIIQTVPDATPECPHQEMDIVFLIDGSGSIDQNDFNQMKGFVQAVMGQFEGTDTLFALMQYSNLLKIHFTFTQFRTSPSQQSLVDPIVQLKGLTFTATGILTVVTQLFHHKNGARKSAKKILIVITDGQKYKDPLEYSDVIPQAEKAGIIRYAIGVGHAFQGPTARQELNTISSAPPQDHVFKVDNFAALGSIQKQLQEKIYAVEGTQSRASSSFQHEMSQEGFSTALTMDGLFLGAVGSFSWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQNLVLGAPRYQHTGKAVIFTQVSRQWRKKAEVTGTQIGSYFGASLCSVDVDSDGSTDLILIGAPHYYEQTRGGQVSVCPLPRGRVQWQCDAVLRGEQGHPWGRFGAALTVLGDVNEDKLIDVAIGAPGEQENRGAVYLFHGASESGISPSHSQRIASSQLSPRLQYFGQALSGGQDLTQDGLMDLAVGARGQVLLLRSLPVLKVGVAMRFSPVEVAKAVYRCWEEKPSALEAGDATVCLTIQKSSLDQLGDIQSSVRFDLALDPGRLTSRAIFNETKNPTLTRRKTLGLGIHCETLKLLLPDCVEDVVSPIILHLNFSLVREPIPSPQNLRPVLAVGSQDLFTASLPFEKNCGQDGLCEGDLGVTLSFSGLQTLTVGSSLELNVIVTVWNAGEDSYGTVVSLYYPAGLSHRRVSGAQKQPHQSALRLACETVPTEDEGLRSSRCSVNHPIFHEGSNGTFIVTFDVSYKATLGDRMLMRASASSENNKASSSKATFQLELPVKYAVYTMISRQEESTKYFNFATSDEKKMKEAEHRYRVNNLSQRDLAISINFWVPVLLNGVAVWDVVMEAPSQSLPCVSERKPPQHSDFLTQISRSPMLDCSIADCLQFRCDVPSFSVQEELDFTLKGNLSFGWVRETLQKKVLVVSVAEITFDTSVYSQLPGQEAFMRAQMEMVLEEDEVYNAIPIIMGSSVGALLLLALITATLYKLGFFKRHYKEMLEDKPEDTATFSGDDFSCVAPNVPLS |
| 预测分子量 | 126,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGaD重组蛋白的3篇参考文献示例(注：内容为模拟示例，实际文献需根据数据库检索确认)：
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1. **标题**: "Expression and Functional Characterization of Recombinant Human Integrin αDβ2 in Mammalian Cells"
**作者**: Smith AB, Jones CD, Lee H
**摘要**: 本研究在HEK293细胞中成功表达并纯化了重组人ITGaD(αD)与β2亚基的异源二聚体。通过流式细胞术和固相结合实验，证实了重组αDβ2整合素可与VCAM-1和ICAM-3结合，揭示了其在白细胞迁移中的潜在作用。
2. **标题**: "Structural Insights into the ITGaD Extracellular Domain by Cryo-EM"
**作者**: Zhang Y, Wang L, Tanaka T
**摘要**: 利用冷冻电镜解析了重组ITGaD胞外域的三维结构，揭示了其与β2亚基的相互作用界面及配体结合的关键氨基酸残基，为靶向αDβ2整合素的药物设计提供了结构基础。
3. **标题**: "Recombinant ITGaD Protein Attenuates Inflammatory Responses in Murine Macrophages"
**作者**: Johnson RM, Kim S, Patel RK
**摘要**: 通过昆虫细胞系统表达可溶性重组ITGaD蛋白，发现其能竞争性抑制巨噬细胞与细胞外基质的粘附，并降低LPS诱导的炎症因子分泌，提示其作为抗炎治疗的潜在工具。
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建议通过PubMed或Google Scholar以关键词“ITGaD recombinant”“integrin alpha D expression”进一步检索真实文献。
**Background of ITGaD Recombinant Protein**
Integrin alpha D (ITGaD), a member of the integrin family, is a transmembrane receptor protein that mediates cell-cell and cell-extracellular matrix interactions. Integrins are heterodimeric proteins composed of alpha (α) and beta (β) subunits, playing critical roles in cell adhesion, migration, signaling, and immune responses. ITGaD specifically pairs with the beta-2 (β2) subunit to form the integrin αDβ2. which is predominantly expressed on leukocytes, including macrophages, neutrophils, and dendritic cells. This integrin is involved in inflammatory processes, immune surveillance, and pathogen clearance by binding ligands such as intercellular adhesion molecules (ICAMs) and fibrinogen.
Recombinant ITGaD protein is engineered through molecular cloning techniques, typically expressed in mammalian systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. The purified protein retains functional domains necessary for ligand binding and interaction with β2 subunits. Researchers utilize ITGaD recombinant proteins to study integrin-mediated signaling pathways, leukocyte adhesion mechanisms, and immune regulation.
In disease contexts, ITGaD has been implicated in chronic inflammatory conditions, atherosclerosis, and cancer metastasis, making it a potential therapeutic target. Recombinant ITGaD enables in vitro assays, structural studies, and antibody development for diagnostic or therapeutic applications. Its role in modulating immune cell infiltration and inflammatory responses underscores its relevance in understanding autoimmune disorders and designing targeted immunotherapies.
Overall, ITGaD recombinant protein serves as a vital tool for dissecting integrin biology and exploring therapeutic strategies in inflammation and immune-related diseases.
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