| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNF4a |
| Uniprot No | P41235 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI |
| 预测分子量 | 59.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNF4α重组蛋白的3篇代表性文献摘要(文献标题和作者为虚拟示例，仅供参考)：
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1. **标题**：*Purification and Functional Analysis of Recombinant HNF4α in DNA Binding Studies*
**作者**：Sladek FM, et al.
**摘要**：研究报道了在大肠杆菌系统中成功表达并纯化重组HNF4α蛋白，通过电泳迁移实验(EMSA)证实其特异性结合靶基因启动子区的DR1元件，揭示了其转录调控机制。
2. **标题**：*Crystal Structure of the Ligand-Binding Domain of HNF4α*
**作者**：Yuan X, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度HNF4α配体结合域(LBD)重组蛋白，通过X射线晶体学解析其三维结构，发现脂肪酸类分子可能作为内源性配体调控其活性。
3. **标题**：*HNF4α Recombinant Protein Restores β-Cell Function in Diabetic Models*
**作者**：Thomas H, et al.
**摘要**：通过哺乳动物细胞表达重组HNF4α蛋白，体外实验表明其可增强胰岛素基因转录，并在糖尿病小鼠模型中验证了其通过靶向递送改善糖代谢的潜力。
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**注**：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar检索关键词“HNF4α recombinant protein”获取最新文献，并优先选择近5年高被引论文。
Hepatocyte nuclear factor 4 alpha (HNF4α), a member of the nuclear receptor superfamily (NR2A1), is a transcription factor critical for organ development and metabolic homeostasis. It is predominantly expressed in the liver, pancreas, and kidneys, where it regulates tissue-specific gene networks. HNF4α exists as multiple isoforms generated through alternative promoter usage and splicing, with HNF4α1 and HNF4α2 being the most studied. Structurally, it contains a DNA-binding domain (DBD) that recognizes specific promoter elements (e.g., DR1 motifs) and a ligand-binding domain (LBD) involved in dimerization and co-regulator interactions. Unlike some nuclear receptors, HNF4α binds constitutively to DNA and does not require exogenous ligands for activation, though its activity can be modulated by post-translational modifications and phospholipid interactions.
Recombinant HNF4α proteins are commonly produced in bacterial (e.g., E. coli) or mammalian expression systems for functional studies. These proteins retain the ability to bind DNA and activate transcription, making them essential tools for studying gene regulation mechanisms. Researchers use them in electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and cell-based reporter assays to dissect HNF4α-dependent pathways. Mutations in HNF4α are linked to maturity-onset diabetes of the young (MODY1) and hepatocellular carcinoma, driving interest in recombinant forms for drug screening and disease modeling. Additionally, engineered variants (e.g., tagged or truncated forms) facilitate protein interaction studies and structural analyses. Its role in maintaining hepatic differentiation and regulating drug-metabolizing enzymes (e.g., CYP450 family) underscores its relevance in pharmacology and toxicology research.
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