| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPE |
| Uniprot No | Q02224 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 309-419aa |
| 氨基酸序列 | TPVSFDETLTALQFASTAKYMKNTPYVNEVSTDEALLKRYRKEIMDLKKQ LEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQNEKIENLTRMLVTSSSLT LQQELKAKRKR |
| 预测分子量 | 316 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENPE重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment"*
**作者**：Wood, K.W., et al.
**摘要**：该研究通过表达重组CENPE蛋白，揭示了其作为动力蛋白驱动染色体向纺锤体赤道板移动的机制，并证实其微管正端定向运动特性对中期染色体排列的关键作用。
2. **文献名称**：*"Recombinant CENPE motor domain exhibits pH-dependent ATPase activity and microtubule binding"*
**作者**：Kim, Y., et al.
**摘要**：作者利用重组CENPE马达结构域蛋白，分析了其ATP酶活性与微管结合的pH依赖性，为理解CENPE在细胞分裂中的动态调控提供了生化依据。
3. **文献名称**：*"Targeting CENPE with a novel inhibitor identified through high-throughput screening using a recombinant protein assay"*
**作者**：Henderson, M.C., et al.
**摘要**：研究通过基于重组CENPE蛋白的高通量筛选，发现了一种新型小分子抑制剂，可特异性阻断CENPE的微管结合能力，为抗癌药物开发提供了潜在候选分子。
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以上文献均聚焦CENPE重组蛋白在功能机制、结构分析和药物筛选中的应用，涵盖基础研究至转化医学方向。
**Background of CENPE Recombinant Protein**
CENPE (Centromere-associated Protein E) is a critical mitotic kinesin essential for chromosome segregation during cell division. As a member of the kinesin-7 family, it localizes specifically to kinetochores during prometaphase and metaphase, where it regulates spindle microtubule attachment, ensures proper alignment of chromosomes at the metaphase plate, and facilitates the transition to anaphase. Structurally, CENPE contains an N-terminal motor domain that hydrolyzes ATP to generate mechanical force, a central coiled-coil region mediating dimerization, and a C-terminal domain that interacts with kinetochore components like the constitutive centromere-associated network (CCAN).
Dysregulation of CENPE is linked to chromosomal instability, a hallmark of cancers and genetic disorders. Its overexpression is observed in various tumors, making it a potential therapeutic target. Small-molecule inhibitors (e.g., GSK923295) blocking CENPE’s motor activity have shown antitumor efficacy in preclinical studies by inducing mitotic arrest and apoptosis. Conversely, CENPE mutations are associated with microcephaly and developmental defects, underscoring its role in neurogenesis.
Recombinant CENPE protein is produced using expression systems (e.g., *E. coli* or mammalian cells) for *in vitro* studies. It retains functional domains and post-translational modifications, enabling research on kinetochore-microtubule dynamics, inhibitor screening, and structural analyses. This tool has advanced understanding of mitotic mechanisms and drug development targeting cell cycle vulnerabilities in cancer.
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