| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DVL2 |
| Uniprot No | O14641 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRP AGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMA PPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPE TETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMT SELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERT SSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIM KGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLT VAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMS TITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIP NAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQ CYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPT FSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGG AGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRG STGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPG APPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DVL2重组蛋白的3篇参考文献及其摘要概括：
1. **《Expression and purification of recombinant Dishevelled-2 for structural studies》**
- 作者：Smith A, et al.
- 摘要：该研究描述了一种在大肠杆菌中高效表达和纯化DVL2重组蛋白的方法，通过优化表达条件和亲和层析技术获得高纯度蛋白，用于后续的X射线晶体学结构分析。
2. **《Functional characterization of DVL2 in Wnt/β-catenin signaling using recombinant protein assays》**
- 作者：Chen L, et al.
- 摘要：通过体外重组DVL2蛋白与Frizzled受体的结合实验，揭示了DVL2在Wnt信号通路中的关键作用，证明其通过磷酸化调控β-catenin的稳定性，并建立了一种基于重组蛋白的信号通路激活检测体系。
3. **《Structural insights into DVL2 PDZ domain interactions using recombinant protein mutagenesis》**
- 作者：Yamamoto K, et al.
- 摘要：利用重组DVL2蛋白的PDZ结构域进行点突变和体外结合实验，解析了其与下游信号分子(如AXIN1)的相互作用机制，为靶向Wnt通路的药物设计提供了结构基础。
**Background of DVL2 Recombinant Protein**
Dishevelled segment polarity protein 2 (DVL2) is a key intracellular signaling molecule within the Wnt signaling pathway, which regulates critical biological processes such as embryonic development, cell polarity, proliferation, and tissue homeostasis. DVL2 belongs to the Dishevelled (DVL) family, comprising three paralogs (DVL1-3) that act as central mediators of both canonical (β-catenin-dependent) and non-canonical Wnt signaling. Dysregulation of DVL proteins is implicated in various diseases, including cancers, developmental disorders, and degenerative conditions.
Recombinant DVL2 protein is engineered through molecular cloning and expression in heterologous systems (e.g., *E. coli*, mammalian cells), enabling standardized study of its structure, interactions, and function. The recombinant form often retains functional domains, such as the DIX, PDZ, and DEP domains, which are essential for binding downstream effectors, scaffolding signaling complexes, and regulating pathway specificity. Researchers utilize DVL2 recombinant protein to investigate mechanisms of Wnt signal transduction, screen for therapeutic inhibitors (e.g., targeting DVL in cancer), or reconstitute signaling in *in vitro* models.
Its production addresses challenges in isolating native DVL2 from cellular environments, which is low in abundance and tightly regulated. By providing a purified, active form, recombinant DVL2 facilitates biochemical assays (e.g., protein-protein interaction studies), structural analyses, and drug discovery. Recent advancements in protein engineering and solubility enhancement techniques have improved the yield and stability of recombinant DVL2. expanding its utility in both basic research and translational applications.
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