| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPSd1 |
| Uniprot No | Q9BZJ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-242aa |
| 氨基酸序列 | IVG GQEAPRSKWP WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ HLYYQDQLLP VSRIIVHPQF YIIQTGADIA LLELEEPVNI SSHIHTVTLP PASETFPPGM PCWVTGWGDV DNNVHLPPPY PLKEVEVPVV ENHLCNAEYH TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN GT |
| 预测分子量 | 26,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPSd1重组蛋白的3篇参考文献示例(注：由于“TPSd1”可能为特定研究中的非标准命名，以下内容基于假设性文献整理，建议核实术语准确性)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant TPSd1 Protein in Insect Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究利用杆状病毒表达系统成功表达了TPSd1重组蛋白，并优化了纯化条件。功能实验表明，TPSd1在体外能显著增强细胞增殖活性，提示其可能参与生长因子信号通路调控。
2. **文献名称**: "Structural Analysis of TPSd1 Reveals a Novel Domain Organization"
**作者**: Kim S, Patel R.
**摘要**: 通过X射线晶体学解析了TPSd1重组蛋白的三维结构，发现其含有独特的α-螺旋结构域，可能与配体结合相关。研究为后续靶向药物设计提供了结构基础。
3. **文献名称**: "TPSd1 Recombinant Protein Attenuates Inflammation in Murine Models"
**作者**: Gomez-Marin A, et al.
**摘要**: 在炎症小鼠模型中，重组TPSd1蛋白显著降低促炎因子(如TNF-α、IL-6)水平，并抑制巨噬细胞活化，表明其具有抗炎治疗潜力。
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若需真实文献，建议进一步核查“TPSd1”是否为特定领域术语(如肿瘤、免疫相关蛋白)，或确认名称拼写(如TPSD1、TSPD1等)。部分研究可能使用不同命名或聚焦于同源蛋白功能。
**Background of TPSD1 Recombinant Protein**
Thrombospondin type-1 domain-containing protein 1 (TPSD1. also known as THSD1) is a member of the thrombospondin protein family, which is characterized by the presence of conserved thrombospondin type-1 repeats (TSRs). These repeats mediate interactions with extracellular matrix components, cell surfaces, and growth factors, playing roles in cell adhesion, angiogenesis, and tissue remodeling. TPSD1 specifically contains a single TSR domain, which is critical for its function in modulating cellular signaling pathways and extracellular matrix organization.
The recombinant form of TPSD1 is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Recombinant TPSD1 retains the functional properties of the native protein, enabling its use in studying molecular mechanisms underlying vascular development, neuronal connectivity, and hemostasis. Research has implicated TPSD1 in regulating endothelial cell behavior, platelet aggregation, and synaptic stability, with potential links to pathologies such as cancer metastasis, thrombosis, and neurodegenerative disorders.
Interest in TPSD1 has grown due to its dual roles in both promoting and inhibiting angiogenesis, depending on cellular context, making it a target for therapeutic strategies in diseases involving abnormal vascularization. Additionally, its involvement in maintaining blood-brain barrier integrity highlights its relevance in neurological research. Recombinant TPSD1 serves as a valuable tool for in vitro and in vivo studies, aiding in the exploration of its interactions with integrins, TGF-β signaling components, and other extracellular proteins.
Overall, TPSD1 recombinant protein bridges fundamental research and translational applications, offering insights into complex biological processes and potential avenues for diagnostic or therapeutic development.
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