| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMC3 |
| Uniprot No | Q9UQE7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1217aa |
| 氨基酸序列 | MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPKFNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDLEKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFYHIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKEHMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG |
| 预测分子量 | 141 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMC3重组蛋白的3篇参考文献(虚构示例，供参考)：
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1. **文献名称**: *Structural Insights into SMC3 Recombinant Protein in Cohesin Complex Assembly*
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组表达和纯化人源SMC3蛋白，结合冷冻电镜技术解析了其与RAD21亚基的复合体结构，揭示了SMC3在染色体黏连蛋白复合体组装中的构象变化及ATP结合位点的关键作用。
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2. **文献名称**: *Functional Characterization of Recombinant SMC3 in DNA Repair Pathways*
**作者**: Patel R, et al.
**摘要**: 利用大肠杆菌表达系统获得高纯度SMC3重组蛋白，实验证明其通过与SMC1A相互作用调控DNA损伤修复，并发现磷酸化修饰影响其在基因组稳定性中的功能。
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3. **文献名称**: *SMC3 Recombinant Protein as a Biomarker in Cancer Cell Lines*
**作者**: Kim H, et al.
**摘要**: 研究通过哺乳动物细胞系表达SMC3重组蛋白，发现其在结直肠癌细胞中异常表达，并验证了其作为潜在治疗靶点的可能性，为癌症精准治疗提供新思路。
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(注：以上文献为示例，实际引用需根据真实研究调整。)
SMC3 (Structural Maintenance of Chromosomes 3) is a critical component of the cohesin complex, a multi-subunit protein assembly essential for chromosome organization, segregation, and DNA repair in eukaryotic cells. The cohesin complex forms a ring-like structure that mediates sister chromatid cohesion during mitosis and meiosis, ensuring accurate chromosome segregation. SMC3. along with SMC1. RAD21. and STAG proteins, constitutes the core of this complex, where SMC3’s ATPase activity and dimerization with SMC1 are vital for cohesin’s dynamic loading onto chromatin.
Recombinant SMC3 refers to the engineered version of the protein produced in heterologous expression systems, such as *E. coli* or mammalian cell lines. This approach allows large-scale production of highly pure, functional SMC3 for biochemical and structural studies. Recombinant technology enables site-specific modifications (e.g., mutations, tags) to investigate molecular mechanisms, protein interactions, or post-translational modifications (e.g., acetylation, phosphorylation) that regulate cohesin dynamics.
Research on recombinant SMC3 has advanced understanding of cohesin’s roles beyond sister chromatid cohesion, including loop extrusion in 3D genome organization, DNA damage repair, and transcriptional regulation. Dysregulation of SMC3 is linked to developmental disorders (e.g., Cornelia de Lange syndrome) and cancers, highlighting its biomedical relevance. Recombinant proteins also facilitate drug screening for therapies targeting cohesinopathies or malignancies with cohesin mutations. Overall, recombinant SMC3 serves as a pivotal tool for dissecting cohesin biology and its implications in disease.
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