| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCSK2 |
| Uniprot No | P16519 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 110-638aa |
| 氨基酸序列 | G YRDINEIDIN MNDPLFTKQW YLINTGQADG TPGLDLNVAE AWELGYTGKG VTIGIMDDGI DYLHPDLASN YNAEASYDFS SNDPYPYPRY TDDWFNSHGT RCAGEVSAAA NNNICGVGVA YNSKVAGIRM LDQPFMTDII EASSISHMPQ LIDIYSASWG PTDNGKTVDG PRELTLQAMA DGVNKGRGGK GSIYVWASGD GGSYDDCNCD GYASSMWTIS INSAINDGRT ALYDESCSST LASTFSNGRK RNPEAGVATT DLYGNCTLRH SGTSAAAPEA AGVFALALEA NLGLTWRDMQ HLTVLTSKRN QLHDEVHQWR RNGVGLEFNH LFGYGVLDAG AMVKMAKDWK TVPERFHCVG GSVQDPEKIP STGKLVLTLT TDACEGKENF VRYLEHVQAV ITVNATRRGD LNINMTSPMG TKSILLSRRP RDDDSKVGFD KWPFMTTHTW GEDARGTWTL ELGFVGSAPQ KGVLKEWTLM LHGTQSAPYI DQVVRDYQSK LAMSKKEELE EELDEAVERS LKSILNKN |
| 预测分子量 | 70,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCSK2重组蛋白的参考文献示例(注：内容为模拟概括，实际文献需通过数据库检索确认)：
1. **"Expression and purification of recombinant human proprotein convertase 2 (PCSK2) in insect cells"**
*作者：Smith J, et al.*
**摘要**：该研究通过杆状病毒-昆虫细胞系统成功表达并纯化了具有活性的重组人PCSK2蛋白，分析了其酶切底物特异性，为研究其在激素前体加工中的功能提供了工具。
2. **"Structural insights into PCSK2 activation and substrate binding using cryo-EM"**
*作者：Li X, Zhang R.*
**摘要**：通过冷冻电镜技术解析了重组PCSK2的构象变化，揭示了其与底物结合的关键结构域，阐明了pH依赖性激活机制。
3. **"Functional characterization of PCSK2 in pancreatic β-cells using recombinant protein overexpression"**
*作者：Garcia M, et al.*
**摘要**：利用重组PCSK2在β细胞中过表达模型，证明其通过调控前胰岛素原加工影响胰岛素分泌，提示其在糖尿病病理中的潜在作用。
4. **"Development of a high-throughput assay for PCSK2 inhibitors using recombinant enzyme"**
*作者：Wang H, et al.*
**摘要**：基于重组PCSK2蛋白建立了荧光底物酶活检测体系，用于筛选特异性抑制剂，为治疗PCSK2异常相关疾病提供药物开发平台。
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建议通过PubMed或Google Scholar以“PCSK2 recombinant expression”“proprotein convertase 2 purification”等关键词检索最新文献，并筛选涉及重组蛋白制备、功能或应用的研究。
PCSK2 (proprotein convertase subtilisin/kexin type 2), also known as prohormone convertase 2 (PC2), is a calcium-dependent serine protease belonging to the proprotein convertase family. This enzyme plays a critical role in the proteolytic processing of prohormones and neuropeptide precursors into their biologically active forms. It is primarily expressed in neuroendocrine tissues, including pancreatic islets, brain, and endocrine glands, where it cleaves precursor proteins at specific paired basic residues (e.g., Lys-Arg or Arg-Arg). Key substrates include proinsulin, proglucagon, proopiomelanocortin (POMC), and other neuropeptides essential for glucose homeostasis, energy balance, and neuronal signaling.
Recombinant PCSK2 protein is engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its enzymatic activity, substrate specificity, and regulatory mechanisms. Its production typically involves cloning the PCSK2 gene into expression vectors, followed by purification via affinity tags. The recombinant form retains the functional domains of the native enzyme, including the catalytic domain, P-domain, and calcium-binding sites, enabling in vitro studies without requiring tissue extraction.
Research on recombinant PCSK2 has advanced understanding of its role in diabetes and neuroendocrine disorders. For instance, PCSK2 knockout mice exhibit impaired processing of proinsulin and proglucagon, leading to glucose intolerance. Additionally, dysregulation of PCSK2 has been linked to obesity, neurodegenerative diseases, and certain cancers. Unlike PCSK9 (a related convertase targeting LDL receptor degradation), PCSK2 is not directly involved in cholesterol metabolism but remains a potential therapeutic target for metabolic syndromes. Current applications include drug screening for enzyme inhibitors/activators and structural studies to elucidate substrate-enzyme interactions. Ongoing efforts aim to harness its regulatory functions for peptide-based therapeutics and personalized medicine approaches.
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