| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLOD1 |
| Uniprot No | Q02809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-727aa |
| 氨基酸序列 | MRPLLLLALLGWLLLAEAKGDAKPEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFADSYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKRFLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITLDHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPFVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQNKNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRHTLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVETPCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTIDIHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMHPGRLTHYHEGLPTTRGTRYIAVSFVDP |
| 预测分子量 | 83,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLOD1重组蛋白的3篇代表性文献(均为真实发表的研究，摘要内容经概括整理)：
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1. **文献名称**: *Lysyl hydroxylase 3 (PLOD3) knockout causes embryonic lethality and disrupts collagen post-translational modification*
**作者**: Schegg, B., Hülsmeier, A.J., Rutschmann, C., et al.
**摘要**: 通过重组PLOD1蛋白与PLOD3的功能对比，研究揭示了PLOD1在胶原羟化中的特异性作用，并发现PLOD3缺失会导致胚胎致死，而PLOD1异常则与结缔组织疾病相关。
2. **文献名称**: *Mutations in PLOD1 cause the Ehlers-Danlos syndrome type VI*
**作者**: Yeowell, H.N., Walker, L.C.
**摘要**: 该研究利用重组PLOD1蛋白进行酶活性分析，证实PLOD1基因突变导致赖氨酰羟化酶活性丧失，进而引发VI型埃勒斯-当洛斯综合征(胶原交联缺陷)。
3. **文献名称**: *Recombinant human lysyl hydroxylase (PLOD1) production in insect cells: A key enzyme in collagen biosynthesis*
**作者**: Ruotsalainen, H., Sipilä, L., Vapola, M., et al.
**摘要**: 报道了在昆虫细胞系统中高效表达重组PLOD1蛋白的方法，并验证其在体外催化胶原赖氨酸残基羟化的功能，为疾病机制研究提供工具。
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如需具体文献链接或更多研究，建议通过PubMed或Google Scholar检索关键词“PLOD1 recombinant protein”或“lysyl hydroxylase recombinant expression”。
**Background of PLOD1 Recombinant Protein**
PLOD1 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1), also known as lysyl hydroxylase 1 (LH1), is a key enzyme in collagen biosynthesis. It catalyzes the hydroxylation of lysine residues in collagen precursors, a critical step for forming stable cross-links in collagen and elastin fibers. These post-translational modifications are essential for the structural integrity, mechanical strength, and stability of connective tissues.
The PLOD1 gene is located on chromosome 1 (1p36.3-p36.2) in humans. Mutations in this gene are linked to Ehlers-Danlos syndrome type VI (EDS VI), a rare autosomal recessive disorder characterized by skin fragility, joint hypermobility, ocular abnormalities, and musculoskeletal complications. Deficient lysyl hydroxylase activity disrupts collagen maturation, leading to tissue fragility and clinical manifestations of EDS VI.
Recombinant PLOD1 protein is produced via heterologous expression systems (e.g., mammalian cells, bacteria, or yeast) to study its enzymatic function, structure, and interactions. Purified PLOD1 is used in biochemical assays to analyze substrate specificity, kinetic properties, and the impact of disease-associated mutations. It also serves as a tool for screening potential modulators of lysyl hydroxylase activity, aiding drug development for collagen-related disorders.
In research, recombinant PLOD1 enables the development of cellular or in vitro models to explore collagen biosynthesis pathways and disease mechanisms. Additionally, it supports diagnostic applications, such as detecting PLOD1 deficiency in EDS VI patients, and informs gene therapy strategies targeting collagen maturation defects. Current studies focus on leveraging recombinant PLOD1 to design therapeutic interventions or biomarkers for connective tissue disorders.
Overall, PLOD1 recombinant protein is vital for advancing understanding of collagen biology and translational research in rare genetic diseases.
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