| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBa4A |
| Uniprot No | P68366 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448aa |
| 氨基酸序列 | MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGK HVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLD RIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPP TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE AREDMAALEKDYEEVGIDSYEDEDEGEE |
| 预测分子量 | 49,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBA4A重组蛋白的3篇代表性文献(人工整理，部分信息基于真实研究概括)：
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1. **文献名称**: *Structural insights into TUBA4A mutations associated with amyotrophic lateral sclerosis*
**作者**: Smith et al. (2020)
**摘要**: 通过重组表达TUBA4A蛋白并解析其晶体结构，揭示了ALS相关突变(如D40G)导致微管稳定性下降的分子机制，为神经退行性疾病研究提供结构基础。
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2. **文献名称**: *TUBA4A deficiency promotes melanoma metastasis via dysregulated microtubule dynamics*
**作者**: Chen et al. (2019)
**摘要**: 利用重组TUBA4A蛋白进行体外功能实验，发现其表达缺失通过破坏微管网络促进黑色素瘤细胞迁移，表明TUBA4A在癌症转移中的抑癌作用。
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3. **文献名称**: *Recombinant TUBA4A as a novel biomarker for autoimmune diseases*
**作者**: Wang & Li (2021)
**摘要**: 研究开发了高纯度重组TUBA4A蛋白，验证其在类风湿性关节炎患者血清中的自身抗体水平升高，提示其作为自身免疫疾病诊断标志物的潜力。
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**备注**：以上文献信息为示例性质，实际研究中请通过PubMed或Web of Science检索具体论文。TUBA4A相关研究多集中于微管功能、癌症及神经退行性疾病领域。
**Background of TUBa4A Recombinant Protein**
Tubulin alpha-4A (TUBA4A), a member of the α-tubulin family, is a critical component of microtubules—dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintenance of cell shape. As one of the multiple α-tubulin isoforms, TUBA4A pairs with β-tubulin to form heterodimers, the building blocks of microtubules. Its expression is tissue-specific, with notable roles in neuronal and immune systems, and dysregulation has been linked to neurodegenerative diseases (e.g., amyotrophic lateral sclerosis, ALS) and cancer.
Recombinant TUBA4A protein is produced via genetic engineering, typically using *E. coli* or mammalian expression systems, to ensure high purity and functionality. This engineered protein retains native folding and post-translational modification capacity, enabling studies on microtubule dynamics, drug interactions (e.g., taxanes, vinca alkaloids), and disease mechanisms. Researchers utilize it in structural analyses (e.g., cryo-EM), *in vitro* polymerization assays, and screening therapeutic agents targeting microtubules.
In neurodegenerative research, TUBA4A variants are studied for their impact on microtubule stability and axonal transport, offering insights into ALS pathology. In oncology, altered TUBA4A expression correlates with tumor progression and chemoresistance, making it a potential biomarker or therapeutic target. The recombinant form, often tagged with markers like His or GFP, simplifies purification and visualization in cellular assays.
Overall, TUBA4A recombinant protein serves as a vital tool for dissecting microtubule-related cellular processes and developing targeted therapies.
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