| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNa11 |
| Uniprot No | P29992 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 氨基酸序列 | MTLESMMACCLSDEVKESKRINAEIEKQLRRDKRDARRELKLLLLGTGES GKSTFIKQMRIIHGAGYSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKIL YKYEQNKANALLIREVDVEKVTTFEHQYVSAIKTLWEDPGIQECYDRRRE YQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPTTGIIEYPFDLENII FRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEE SKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQR DAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQ LNLKEYNLV |
| 预测分子量 | 42,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于G蛋白α亚基研究的常见方向构造的示例参考文献(仅供参考，建议通过学术数据库核实真实文献)：
1. **文献名称**: "Functional Characterization of Recombinant GNA11 Protein in Calcium-Sensing Receptor Signaling"
**作者**: Smith A, et al.
**摘要**: 本研究通过杆状病毒系统表达重组GNA11蛋白，分析其与钙敏感受体(CaSR)的偶联机制。发现GNA11在介导细胞内钙信号传导中起关键作用，并揭示了特定突变体导致信号异常与疾病的相关性。
2. **文献名称**: "Structural Analysis of Gα11 in Complex with GDP/AlF4− Using X-ray Crystallography"
**作者**: Lee JH, et al.
**摘要**: 利用重组GNA11蛋白进行结晶学研究，解析了其与GDP/AlF4−复合体的三维结构，揭示了Gα11的活化构象变化，为靶向G蛋白的药物设计提供结构基础。
3. **文献名称**: "Role of GNA11 Mutations in Hypocalciuric Hypercalcemia: Insights from Recombinant Protein Assays"
**作者**: Brown KE, et al.
**摘要**: 通过体外重组表达野生型及突变型GNA11蛋白，证实特定错义突变(如R181Q)可导致G蛋白持续激活，解释了家族性低钙尿性高钙血症的分子机制。
4. **文献名称**: "Development of a High-Yield E. coli Expression System for GNA11 Recombinant Protein Production"
**作者**: Zhang Q, et al.
**摘要**: 优化大肠杆菌表达体系，实现可溶性GNA11蛋白的高效纯化，并验证其与Gβγ亚基的体外重组功能，为大规模筛选G蛋白调控剂奠定技术基础。
**注意事项**：
- 以上文献为示例性质，实际研究中请通过PubMed/Google Scholar检索真实文献(关键词：GNA11 recombinant protein, Gα11 expression, calcium signaling)。
- 真实研究可能涉及GNA11在癌症(如葡萄膜黑色素瘤)、代谢疾病或信号转导通路中的具体作用。
**Background of GNa11 Recombinant Protein**
GNa11. or Guanine Nucleotide-binding protein subunit alpha-11. is a member of the G protein alpha subunit family, which plays a critical role in mediating intracellular signaling cascades triggered by G protein-coupled receptors (GPCRs). Encoded by the *GNA11* gene, this protein is part of the Gq/11 subfamily and functions as a molecular switch, cycling between inactive GDP-bound and active GTP-bound states to regulate downstream effectors like phospholipase C-beta (PLC-β). Activation of GNa11 leads to the hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2), generating secondary messengers (IP3 and DAG) that modulate calcium release and protein kinase C (PKC) signaling.
Recombinant GNa11 protein is engineered through heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research applications. Its recombinant form enables detailed studies of GPCR signaling mechanisms, structure-function relationships, and interactions with regulatory proteins. Mutations in *GNA11* are linked to diseases such as hypocalciuric hypercalcemia type 2 and uveal melanoma, making the recombinant protein valuable for investigating pathogenic variants and screening targeted therapies.
In drug discovery, GNa11 recombinant protein aids in identifying modulators of Gq/11 pathways, which are implicated in cardiovascular, neurological, and oncological disorders. It also serves as an antigen for antibody development and a tool for high-throughput assays. By providing a controlled source of functional protein, recombinant GNa11 enhances our understanding of GPCR biology and supports translational research aimed at therapeutic innovation.
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