| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2aK3 |
| Uniprot No | Q9NZJ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1116aa |
| 氨基酸序列 | MERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAVAAAEVTVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGALFQWDQDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKVRYICSALGCRQWDSDEMEQEEDILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIESTFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGHLEWEYQFCTPIASAWLLKDGKVIPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPSSPKALESVTNENAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRSTQITVRFLDNPHYNKNIRKKDPVLLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESETQCQTENKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFATKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN |
| 预测分子量 | 125,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2AK3(PERK)重组蛋白的3篇参考文献示例(文献信息为模拟内容，建议通过学术数据库核实)：
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1. **标题**: *"Expression and functional characterization of recombinant human EIF2AK3 kinase domain in endoplasmic reticulum stress signaling"*
**作者**: Zhang, Y. et al. (2015)
**摘要**: 研究通过大肠杆菌系统成功表达并纯化EIF2AK3的激酶结构域重组蛋白，证实其磷酸化eIF2α的活性，并验证其在体外内质网应激模型中的功能，为靶向药物开发提供基础。
2. **标题**: *"Structural basis of PERK activation by unfolded proteins: Insights from cryo-EM analysis of recombinant EIF2AK3 complexes"*
**作者**: Harding, H.P. et al. (2018)
**摘要**: 利用重组EIF2AK3蛋白进行冷冻电镜结构解析，揭示内质网应激下PERK的激活机制，发现未折叠蛋白直接诱导其寡聚化及自磷酸化，阐明UPR的分子调控路径。
3. **标题**: *"Recombinant EIF2AK3 protein attenuates neuronal apoptosis in Alzheimer's disease models via modulating unfolded protein response"*
**作者**: Chen, L. et al. (2020)
**摘要**: 在阿尔茨海默病细胞和小鼠模型中，外源性重组EIF2AK3蛋白通过调控UPR信号通路显著减少神经元凋亡，提示其在神经退行性疾病治疗中的潜在应用价值。
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**备注**：以上文献为示例，实际引用请通过PubMed、Google Scholar等平台检索真实文献(关键词：EIF2AK3/PKR-like ER kinase, recombinant protein, endoplasmic reticulum stress)。
**Background of EIF2AK3 Recombinant Protein**
EIF2AK3 (Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3), also known as PERK (Protein kinase R-like endoplasmic reticulum kinase), is a serine/threonine kinase critical to the unfolded protein response (UPR) pathway. It is activated under endoplasmic reticulum (ER) stress, a condition caused by the accumulation of misfolded proteins in the ER lumen. EIF2AK3 senses ER stress via its luminal domain, leading to autophosphorylation and dimerization, which subsequently phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α). This phosphorylation attenuates global protein synthesis while selectively promoting the translation of stress-responsive genes, such as ATF4. to restore cellular homeostasis.
Mutations in the *EIF2AK3* gene are linked to Wolcott-Rallison syndrome, a rare autosomal recessive disorder characterized by neonatal diabetes, skeletal dysplasia, and liver dysfunction. Beyond genetic disorders, EIF2AK3 dysregulation is implicated in various pathologies, including diabetes, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where chronic ER stress drives apoptosis or survival mechanisms.
Recombinant EIF2AK3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to study its structural and functional properties, kinase activity, and interactions with substrates or inhibitors. Its applications extend to drug discovery, particularly in targeting ER stress-related diseases. Researchers also employ recombinant EIF2AK3 to model cellular stress responses *in vitro* and evaluate therapeutic strategies aimed at modulating UPR pathways.
Overall, EIF2AK3 recombinant protein serves as a vital tool for elucidating ER stress mechanisms and developing interventions for diseases linked to proteostatic imbalance.
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