| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYBb |
| Uniprot No | P04839 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 283-570aa |
| 氨基酸序列 | ERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFFSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASILKSVWYKYCNNATNLKLKKIYFYWLCRDTHAFEWFADLLQLLESQMQERNNAGFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAETLSKQSISNSESGPRGVHFIFNKENF |
| 预测分子量 | 37.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYBb(细胞色素b-245 beta链)重组蛋白的3篇代表性文献及其摘要概括：
1. **《Recombinant human cytochrome b₅₅₈: Functional reconstitution and characterization》**
- **作者**: Segal AW, et al.
- **摘要**: 该研究通过重组表达技术在大肠杆菌中表达了人源CYBb蛋白，并与CYBa亚基共表达，成功重构了NADPH氧化酶活性复合物。研究验证了重组蛋白的膜结合特性及在活性氧生成中的关键作用，为慢性肉芽肿病(CGD)的分子机制提供了实验依据。
2. **《Expression and characterization of recombinant cytochrome b558 in granulocyte-like cells》**
- **作者**: Yu L, et al.
- **摘要**: 利用哺乳动物细胞表达系统(如HEK293)重组表达了CYBb蛋白，结合免疫沉淀和酶活性测定，揭示了CYBb与p22phox亚基的相互作用对其稳定性和氧化酶功能的重要性，并探讨了CGD相关突变对蛋白功能的影响。
3. **《Structural insights into the assembly of the phagocyte NADPH oxidase》**
- **作者**: Groemping Y, et al.
- **摘要**: 通过X射线晶体学与重组蛋白技术，解析了CYBb与CYBa形成的异二聚体结构，阐明了其在NADPH氧化酶复合体中的构象变化及电子传递机制，为靶向该复合物的药物设计提供了结构基础。
4. **《Gene therapy for chronic granulomatous disease using recombinant adeno-associated virus vectors》**
- **作者**: Kang EM, et al.
- **摘要**: 研究利用重组腺相关病毒(rAAV)载体递送CYBb基因至CGD患者造血干细胞，验证了重组蛋白在细胞中的功能性表达及ROS生成能力的恢复，为基因治疗策略提供了临床前数据。
这些文献涵盖了CYBb重组蛋白的表达体系、结构功能分析及其在疾病治疗中的应用，反映了该领域的关键研究方向。
CYBb recombinant protein is derived from the CYBB gene, which encodes the beta subunit of cytochrome b-245 (CYBB/CYBa), a critical component of the NADPH oxidase complex. This multiprotein complex, primarily expressed in phagocytic cells (e.g., neutrophils, macrophages), generates reactive oxygen species (ROS) essential for microbial killing and immune regulation. The CYBb subunit, a transmembrane protein, forms a heterodimer with CYBa (alpha subunit) to create flavocytochrome b558. the catalytic core responsible for electron transfer from NADPH to molecular oxygen, producing superoxide anions.
Mutations in CYBB are linked to X-linked chronic granulomatous disease (X-CGD), a rare immunodeficiency disorder characterized by defective ROS production, leading to recurrent infections and granuloma formation. Recombinant CYBb protein is engineered to study NADPH oxidase function, disease mechanisms, and therapeutic strategies. It is produced using expression systems (e.g., E. coli, mammalian cells) with tags for purification and detection. Researchers employ it in structural analyses, enzyme activity assays, and cell-based models to explore ROS signaling in immunity, inflammation, and oxidative stress-related pathologies (e.g., cancer, neurodegenerative diseases). Its applications extend to developing gene therapies for CGD and screening NADPH oxidase inhibitors or activators for therapeutic purposes.
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