| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMPK Alpha 1 |
| Uniprot No | Q13131 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559aa |
| 氨基酸序列 | MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC ANLIKILAQ |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMPK Alpha 1重组蛋白的3篇代表性文献：
1. **"Recombinant expression and purification of AMPK α1β1γ1 heterotrimer complexes for structural analysis"**
- **作者**: Xiao, B., et al.
- **摘要**: 该研究描述了在大肠杆菌中重组表达人源AMPK α1、β1和γ1亚基，并通过亲和层析和凝胶过滤纯化获得功能性异源三聚体复合物，为后续晶体结构解析奠定了基础。
2. **"Structural basis for AMPK activation upon energy depletion"**
- **作者**: Chen, L., et al.
- **摘要**: 通过体外重组AMPK α1亚基与β/γ亚基复合物，结合冷冻电镜技术揭示了能量应激下AMPK的构象变化机制，阐明了α1亚基在腺苷酸依赖的激活过程中的关键作用。
3. **"AMPK α1 phosphorylation of DEPTOR controls mTORC1 activity in cancer cells"**
- **作者**: Wang, Z., et al.
- **摘要**: 利用重组AMPK α1蛋白进行体外激酶实验，证实其直接磷酸化mTOR通路抑制蛋白DEPTOR，从而调控细胞生长代谢，揭示了α1亚基在肿瘤代谢中的功能。
注：以上文献信息为示例性质，实际引用需核对原文。如需具体文章，建议通过PubMed或Web of Science以“AMPK alpha 1 recombinant protein”为关键词检索最新研究。
AMP-activated protein kinase (AMPK) Alpha 1 is a critical subunit of the heterotrimeric AMPK complex, a central regulator of cellular energy homeostasis. AMPK functions as a metabolic sensor, activated under low-energy conditions (e.g., ATP depletion, elevated AMP/ADP) to restore energy balance by inhibiting anabolic pathways and promoting catabolic processes like glucose uptake, fatty acid oxidation, and autophagy. The kinase complex consists of a catalytic α-subunit (α1 or α2) and regulatory β- and γ-subunits. The α1 isoform (encoded by *PRKAA1*) is ubiquitously expressed, with prominent roles in liver, skeletal muscle, and other tissues, while α2 is more restricted to stress-responsive organs.
Recombinant AMPK Alpha 1 protein is produced using expression systems (e.g., *E. coli*, insect cells) to enable mechanistic studies of its structure, enzymatic activity, and interactions. The α1 subunit contains a conserved N-terminal kinase domain (residues 1-280) responsible for catalytic activity, a auto-inhibitory domain (AID, residues 313-392), and a C-terminal region mediating binding to β/γ subunits. Recombinant versions often include tags (e.g., GST, His) for purification and may be co-expressed with β/γ subunits to reconstitute functional complexes.
This tool has advanced research in metabolic diseases (e.g., diabetes, obesity), cancer (AMPK modulates cell growth/apoptosis), and aging. It allows in vitro kinase assays to screen activators (e.g., metformin, AICAR) or study post-translational modifications (e.g., phosphorylation at Thr172 by upstream kinases like LKB1). Quality-controlled batches ensure consistency in biochemical studies, structural analyses (crystallography), and drug discovery targeting AMPK pathways. Its recombinant form bypasses challenges in isolating endogenous AMPK, offering high purity and activity for dissecting energy-sensing mechanisms.
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