| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD42b |
| Uniprot No | P07359 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652aa |
| 氨基酸序列 | MPLLLLLLLLPSPLHPHPICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTKEQTTFPPRWTPNFTLHMESITFSKTPKSTTEPTPSPTTSEPVPEPAPNMTTLEPTPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTSEPAPSPTTPEPTPIPTIATSPTILVSATSLITPKSTFLTTTKPVSLLESTKKTIPELDQPPKLRGVLQGHLESSRNDPFLHPDFCCLLPLGFYVLGLFWLLFASVVLILLLSWVGHVKPQALDSGQGAALTTATQTTHLELQRGRQVTVPRAWLLFLRGSLPTFRSSLFLWVRPNGRVGPLVAGRRPSALSQGRGQDLLSTVSIRYSGHSL |
| 预测分子量 | 71,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD42b(GPIbα)重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Structural and functional characterization of recombinant platelet glycoprotein Ibα*
**作者**：López JA et al.
**摘要**：本研究利用哺乳动物表达系统成功表达并纯化重组CD42b蛋白，解析其与血管性血友病因子(vWF)结合的关键结构域，证实重组蛋白在体外可恢复血小板粘附功能，为血小板功能缺陷疾病研究提供工具。
2. **文献名称**：*Expression and functional analysis of recombinant GPIb-IX complex in CHO cells*
**作者**：Dong JF et al.
**摘要**：通过在CHO细胞中共表达CD42b(GPIbα)及其复合物亚基，获得具有生物活性的重组GPIb-IX复合物，证明其与vWF和凝血酶的相互作用，为开发血小板相关疾病的药物筛选平台奠定基础。
3. **文献名称**：*Recombinant CD42b fragments as potential antithrombotic agents*
**作者**：Berndt MC et al.
**摘要**：研究设计了一种截短型CD42b重组蛋白，可竞争性抑制vWF与血小板的结合，显著减少动物模型中的血栓形成，提示其在抗血栓治疗中的潜在应用价值。
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**备注**：以上文献为模拟示例，实际引用请通过PubMed或SciHub检索关键词(如“CD42b recombinant”、“GPIbα expression”)获取全文。
CD42b, also known as glycoprotein Ibα (GPIbα), is a critical subunit of the platelet membrane glycoprotein Ib-IX-V (GPIb-IX-V) complex, which plays a pivotal role in platelet adhesion and thrombus formation. This protein is encoded by the *GPIBA* gene and is predominantly expressed on the surface of platelets and megakaryocytes. CD42b interacts with von Willebrand factor (VWF) in the bloodstream, facilitating platelet attachment to damaged vascular endothelium under high shear stress conditions—a key step in primary hemostasis. Structurally, CD42b contains leucine-rich repeats, a ligand-binding domain, and a mucin-like region, enabling its functional versatility.
Recombinant CD42b protein is engineered using heterologous expression systems, such as mammalian cell lines (e.g., CHO or HEK293), to ensure proper post-translational modifications, including glycosylation, which is essential for ligand-binding activity. Researchers often produce soluble forms of CD42b by truncating its transmembrane domain or fusing it with tags (e.g., Fc or His tags) for purification and detection. This recombinant tool is widely utilized to study platelet activation mechanisms, screen anti-thrombotic drugs, and investigate bleeding disorders like Bernard-Soulier syndrome, which is linked to GPIb-IX-V complex defects. Additionally, it serves as an antigen in immunoassays for autoimmune platelet disorder diagnostics. Quality control involves verifying molecular weight, glycosylation patterns, and binding affinity to VWF or monoclonal antibodies. Its role in thrombosis and inflammation also makes it a potential therapeutic target for cardiovascular diseases.
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