| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1qR1 |
| Uniprot No | Q9NPY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-652aa |
| 氨基酸序列 | MATSMGLLLLLLLLLTQPGAGTGADTEAVVCVGTACYTAHSGKLSAAEAQNHCNQNGGNLATVKSKEEAQHVQRVLAQLLRREAALTARMSKFWIGLQREKGKCLDPSLPLKGFSWVGGGEDTPYSNWHKELRNSCISKRCVSLLLDLSQPLLPSRLPKWSEGPCGSPGSPGSNIEGFVCKFSFKGMCRPLALGGPGQVTYTTPFQTTSSSLEAVPFASAANVACGEGDKDETQSHYFLCKEKAPDVFDWGSSGPLCVSPKYGCNFNNGGCHQDCFEGGDGSFLCGCRPGFRLLDDLVTCASRNPCSSSPCRGGATCVLGPHGKNYTCRCPQGYQLDSSQLDCVDVDECQDSPCAQECVNTPGGFRCECWVGYEPGGPGEGACQDVDECALGRSPCAQGCTNTDGSFHCSCEEGYVLAGEDGTQCQDVDECVGPGGPLCDSLCFNTQGSFHCGCLPGWVLAPNGVSCTMGPVSLGPPSGPPDEEDKGEKEGSTVPRAATASPTRGPEGTPKATPTTSRPSLSSDAPITSAPLKMLAPSGSPGVWREPSIHHATAASGPQEPAGGDSSVATQNNDGTDGQKLLLFYILGTVVAILLLLALALGLLVYRKRRAKREEKKEKKPQNAADSYSWVPERAESRAMENQYSPTPGTDC |
| 预测分子量 | 68,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1qR1(gC1qR/CD93)重组蛋白研究的3篇代表性文献摘要：
1. **"Recombinant gC1qR/p32: A Potential Therapeutic Target in Inflammatory Diseases"**
- **作者**: Ghebrehiwet, B., et al.
- **摘要**: 研究利用大肠杆菌表达重组gC1qR蛋白，验证其与C1q及病原体配体的结合能力，揭示其在补体激活和炎症反应中的调控作用，提出其作为自身免疫疾病治疗靶点的潜力。
2. **"Structural and Functional Characterization of the C-Terminal Domain of gC1qR"**
- **作者**: Jiang, J., et al.
- **摘要**: 通过哺乳动物系统表达gC1qR的C端重组蛋白，结合X射线晶体学解析结构，阐明其与病毒蛋白相互作用的分子机制，为抗病毒药物设计提供结构基础。
3. **"CD93 Recombinant Protein Attenuates Neovascularization in Murine Models"**
- **作者**: Norsworthy, P.J., et al.
- **摘要**: 构建小鼠CD93重组蛋白，实验表明其通过抑制内皮细胞迁移和血管生成信号通路，显著减少肿瘤和视网膜病变模型中的异常血管形成，提示其抗血管生成治疗价值。
如需具体文献标题或补充更多研究，可进一步说明需求方向(如结构、疾病应用等)。
C1q receptor 1 (C1qR1), also known as CD93. is a transmembrane glycoprotein involved in immune regulation and cellular processes such as phagocytosis, inflammation, and angiogenesis. It binds to the globular head regions of C1q, a key component of the complement system, facilitating the clearance of immune complexes and apoptotic cells via opsonization. Structurally, C1qR1 contains a C-type lectin-like domain, a mucin-like region, and a cytoplasmic tail with signaling motifs, enabling interactions with ligands and intracellular pathways. Dysregulation of C1qR1 has been linked to autoimmune diseases, cancer progression, and vascular disorders, highlighting its therapeutic potential.
Recombinant C1qR1 protein is produced using expression systems like mammalian (e.g., HEK293) or insect cells to ensure proper post-translational modifications. This engineered protein retains functional domains required for ligand binding and is purified via affinity chromatography. It serves as a critical tool for studying C1q-mediated immune responses, receptor-ligand interactions, and signaling mechanisms in vitro. Researchers utilize it to screen inhibitors for autoimmune or inflammatory therapies and explore its role in tumor angiogenesis. Additionally, recombinant C1qR1 aids in structural studies (e.g., crystallography) to map binding interfaces and design targeted biologics. Its applications extend to diagnostic assays for diseases associated with complement dysfunction. By providing a standardized, soluble form of the receptor, recombinant C1qR1 accelerates both basic research and drug development in immunology and oncology.
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