| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEP1 |
| Uniprot No | Q12913 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 997-1337aa |
| 氨基酸序列 | RKKRKDAKNNEVSFSQIKPKKSKLIRVENFEAYFKKQQADSNCGFAEEYEDLKLVGISQPKYAAELAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINANYMPGYHSKKDFIATQGPLPNTLKDFWRMVWEKNVYAIIMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSEIVLPEWTIRDFTVKNIQTSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQSPPESPILVHCSAGVGRTGTFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIVRSQKDSKVDLIYQNTTAMTIYENLAPVTTFGKTNGYIA |
| 预测分子量 | 65 Kda |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEP1重组蛋白的3篇示例参考文献(虚构示例，仅供参考)：
1. **《DEP1重组蛋白在植物细胞信号传导中的功能研究》**
作者：张伟等
摘要：本研究通过原核表达系统制备DEP1重组蛋白，证实其参与调控水稻分蘖数及籽粒发育的分子机制，揭示DEP1通过MAPK信号通路影响细胞分裂。
2. **《重组DEP1蛋白抑制肺癌细胞迁移的机制分析》**
作者：Chen L, Wang H
摘要：利用哺乳动物表达系统获得功能性DEP1重组蛋白，实验表明其通过下调EGFR磷酸化抑制A549细胞侵袭转移，为癌症治疗提供新靶点。
3. **《高效可溶性DEP1重组蛋白的毕赤酵母表达优化》**
作者：Kim J et al.
摘要：通过密码子优化和发酵条件调控，在毕赤酵母中实现DEP1蛋白的高效分泌表达，获得高纯度活性蛋白用于体外磷酸酶活性检测。
(注：上述文献为模拟示例，实际研究需通过PubMed/CNKI等平台检索真实文献)
**Background of DEP1 Recombinant Protein**
DEP1 (Density-Enhanced Phosphatase-1), also known as PTPRJ (Protein Tyrosine Phosphatase Receptor Type J), is a transmembrane protein tyrosine phosphatase implicated in regulating cell signaling, proliferation, and apoptosis. It belongs to the receptor-type PTP family, characterized by extracellular fibronectin type III-like domains, a single transmembrane region, and intracellular catalytic phosphatase domains. DEP1 plays a critical role in modulating receptor tyrosine kinase (RTK) signaling, including pathways driven by PDGFR, VEGFR, and EGFR, by dephosphorylating key tyrosine residues to attenuate activation.
The gene encoding DEP1. *PTPRJ*, has been identified as a tumor suppressor in multiple cancers. Loss-of-function mutations or reduced expression of DEP1 are associated with increased cell proliferation, survival, and angiogenesis in malignancies such as colorectal, thyroid, and lung cancers. Conversely, DEP1 overexpression inhibits oncogenic signaling, highlighting its regulatory importance.
Recombinant DEP1 protein is engineered for research and therapeutic exploration. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains enzymatic activity and structural integrity for functional studies. Researchers utilize DEP1 recombinant protein to investigate its phosphatase activity, interaction partners, and role in pathways like MAPK/ERK or PI3K/AKT. Additionally, it serves as a tool for screening potential inhibitors or activators to modulate DEP1-related signaling in disease contexts.
Beyond cancer, DEP1 is studied in metabolic disorders and inflammation due to its involvement in insulin/leptin signaling and immune cell regulation. Its dual role in diverse pathologies underscores DEP1 as a multifunctional regulator, making the recombinant protein a valuable resource for both basic research and drug development.
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