| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGb5 |
| Uniprot No | P18084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-799aa |
| 氨基酸序列 | MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDLYYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPNCVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDVPHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTALIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGDTASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEFGKIYGPFCECDNFSCARNKGVLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQICSERGHCLCGQCQCTEPGAFGEMCEKCPTCPDACSTKRDCVECLLLHSGKPDNQTCHSLCRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELPSGKSNLTVLREPECGNTPNAMTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMASNPLYRKPISTHTVDFTFNKFNKSYNGTVD |
| 预测分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGb5重组蛋白的参考文献示例(内容为模拟，仅供参考)：
1. **标题**：*"Recombinant Integrin β5 Subunit Expression and Its Role in Cell Adhesion"*
**作者**：Smith J, et al.
**摘要**：研究成功在大肠杆菌中表达ITGb5重组蛋白，证实其与αv亚基结合形成功能性整合素，并促进肿瘤细胞迁移和粘附。
2. **标题**：*"Structural Insights into ITGb5 Recombinant Protein and Ligand Binding"*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析ITGb5重组蛋白的3D结构，揭示其与纤维连接蛋白相互作用的分子机制，为靶向药物设计提供基础。
3. **标题**：*"ITGb5 Recombinant Protein Modulates Angiogenesis in Cancer Models"*
**作者**：Lee S, et al.
**摘要**：利用哺乳动物细胞表达的ITGb5重组蛋白，证明其在肿瘤血管生成中的关键作用，并验证其通过TGF-β通路调控内皮细胞增殖。
4. **标题**：*"Functional Characterization of Recombinant ITGb5 in Apoptosis Regulation"*
**作者**：Brown K, et al.
**摘要**：研究发现ITGb5重组蛋白通过激活caspase信号通路参与上皮细胞凋亡，提示其在组织修复和纤维化疾病中的潜在应用。
(注：以上文献信息为示例性模拟，实际引用请查询真实数据库。)
Integrin beta-5 (ITGβ5) is a transmembrane receptor subunit that plays a critical role in cell adhesion, signaling, and communication with the extracellular matrix (ECM). As a member of the integrin family, ITGβ5 pairs exclusively with the alpha-v (αv) subunit to form the heterodimeric αvβ5 integrin complex. This receptor recognizes arginine-glycine-aspartic acid (RGD) motifs present in ECM proteins such as vitronectin, fibronectin, and osteopontin, facilitating cell-ECM interactions. The αvβ5 integrin is notably involved in mediating cell migration, proliferation, and survival through activation of downstream signaling pathways, including focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K)/Akt.
ITGβ5 has garnered attention for its role in pathological processes, particularly cancer progression and fibrosis. In oncology, αvβ5 overexpression is linked to tumor angiogenesis, metastasis, and resistance to apoptosis, making it a potential therapeutic target. Additionally, ITGβ5 participates in TGF-β1 activation, a key pathway in fibrosis and tissue remodeling. Dysregulation of ITGβ5-mediated signaling has also been implicated in inflammatory diseases and wound healing disorders.
Recombinant ITGβ5 protein is engineered for research and therapeutic applications, typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. It retains functional epitopes for ligand binding and interaction with αv, enabling studies on integrin-mediated mechanisms. Researchers use ITGβ5 recombinant proteins to investigate cell adhesion dynamics, screen inhibitory drugs, or develop targeted therapies. Its soluble form may also serve as a decoy receptor to block pathological αvβ5 interactions. Overall, ITGβ5 recombinant proteins are vital tools for dissecting integrin biology and advancing translational research in cancer, fibrosis, and regenerative medicine.
×
