| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLNa |
| Uniprot No | Q96C61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-838aa |
| 氨基酸序列 | MPSGKVAQPTITDNKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFY VDYVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAE ISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMR MSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGI SFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHE GHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPT EPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSH CDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGT HTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEF SIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSV KFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNG AKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGT HIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGA GALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGG SPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASK VVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHV GSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRVVVP |
| 预测分子量 | 118 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLNa重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Filamin A mechanosensor: Mechanisms of structural regulation and interdomain communication*
**作者**：Nakamura F, et al.
**摘要**：研究利用重组FLNa蛋白片段解析其结构域间的机械传感机制，揭示机械力如何调控FLNa与肌动蛋白及整合素等配体的结合，阐明其介导细胞骨架动态重塑的分子基础。
2. **文献名称**：*Structural basis of filamin A function in cell adhesion and migration*
**作者**：Zhou X, et al.
**摘要**：通过重组表达FLNa的Ig-like结构域，发现其第23-24结构域特异性结合整合素β胞内段，揭示FLNa通过桥接细胞膜受体与细胞骨架调控细胞迁移的分子机制。
3. **文献名称**：*Disease-associated mutations disrupt the dimerization and cytoskeletal function of filamin A*
**作者**：Robertson SP, et al.
**摘要**：利用重组FLNa突变体证明，额叶颅骨发育不良相关突变导致其二聚化能力受损，削弱肌动蛋白交联功能，进而影响细胞力学信号传导，阐释致病机理。
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注：以上文献信息为示例性质，实际引用时请核实具体论文的准确性。
FLNa (Filamin A) recombinant protein is a engineered form of the cytoskeletal protein Filamin A, widely used in biochemical and biomedical research. Native FLNa, encoded by the FLNA gene, acts as an actin-crosslinking scaffold that regulates cell mechanics, signaling, and adhesion. Its structure includes an N-terminal actin-binding domain, 24 immunoglobulin-like repeats facilitating protein interactions, and a dimerization domain. FLNa interacts with over 90 binding partners, including integrins, ion channels, and membrane receptors, making it crucial for cell migration, mechanotransduction, and tissue development.
Recombinant FLNa proteins are typically produced in eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. Researchers engineer specific domains or full-length variants to study FLNa's role in diseases like X-linked periventricular heterotopia (caused by FLNA mutations), cancer metastasis, and cardiovascular disorders. Common applications include in vitro binding assays to map interaction networks, structural studies using cryo-EM or X-ray crystallography, and functional analyses of disease-associated mutations.
The recombinant form enables controlled experiments to decipher how FLNa coordinates cellular responses to mechanical forces and biochemical signals. Its utility extends to drug discovery screens targeting FLNa-mediated pathways in tumor invasion or vascular abnormalities. As an essential protein linking extracellular matrix sensing to intracellular signaling, recombinant FLNa remains vital for understanding cell behavior in health and disease.
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