| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR0B2 |
| Uniprot No | Q15466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257aa |
| 氨基酸序列 | MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLC APHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGL AQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQ CCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLC EVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLL GDMLLLR |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NR0B2(SHP)重组蛋白的3篇代表性文献，按作者、文献名称和摘要内容分类整理：
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### 1. **文献名称**: *"Structural and functional characterization of the human nuclear receptor SHP ligand-binding domain"*
**作者**: Zhang Y. et al. (2005)
**摘要**: 该研究通过重组技术表达并纯化了人源NR0B2(SHP)的配体结合域(LBD)，利用X射线晶体学解析其三维结构。结果表明SHP缺乏典型的配体结合口袋，其抑制功能依赖于与其它核受体(如LRH-1)的相互作用，揭示了其作为转录抑制因子的分子机制。
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### 2. **文献名称**: *"Recombinant SHP modulates bile acid homeostasis through interaction with LRH-1 and FXR"*
**作者**: Båvner A. et al. (2002)
**摘要**: 研究利用重组SHP蛋白分析其在胆汁酸合成中的调控作用。实验表明，重组SHP通过与LRH-1和FXR结合，抑制CYP7A1等关键基因的转录，从而负反馈调节胆汁酸代谢通路。该研究为SHP的生理功能提供了体外实验支持。
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### 3. **文献名称**: *"Functional dissection of SHP nuclear receptor in metabolic pathways using recombinant protein mutants"*
**作者**: Johansson L. et al. (2003)
**摘要**: 通过构建重组SHP蛋白的多种突变体(如磷酸化位点突变)，发现其转录抑制活性依赖于特定的结构域(如LBD和抑制模块)。研究还表明，重组SHP可抑制ERRγ等核受体的活性，提示其在糖脂代谢中的广泛调控作用。
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### 简要说明：
以上文献均围绕NR0B2重组蛋白的表达、结构解析及功能验证展开，涵盖其与代谢相关核受体的相互作用机制、结构功能关系及病理生理意义。如需扩展，可进一步检索关键词“NR0B2/SHP recombinant protein expression”或结合具体研究领域筛选。
**Background of NR0B2 Recombinant Protein**
NR0B2 (Nuclear Receptor Subfamily 0 Group B Member 2), also known as Small Heterodimer Partner (SHP), is an atypical member of the nuclear receptor superfamily. Unlike most nuclear receptors, NR0B2 lacks a DNA-binding domain and functions primarily as a transcriptional regulator by interacting with other nuclear receptors, transcription factors, and co-regulators. It plays a critical role in metabolic homeostasis, including bile acid synthesis, cholesterol metabolism, and glucose regulation, by repressing or enhancing the activity of receptors such as FXR (Farnesoid X Receptor), LRH-1 (Liver Receptor Homolog-1), and HNF4α (Hepatocyte Nuclear Factor 4α).
Recombinant NR0B2 protein is engineered for in vitro studies to elucidate its molecular interactions and regulatory mechanisms. Produced using expression systems like *E. coli* or mammalian cells, the purified protein retains its functional domains, including the ligand-binding domain (LBD) that mediates receptor interactions. Researchers utilize this protein to investigate its role in gene silencing, co-repressor recruitment, and metabolic pathway modulation.
NR0B2 dysregulation is linked to metabolic disorders (e.g., obesity, diabetes), liver diseases, and cancers, making its recombinant form valuable for drug discovery. Structural studies using recombinant NR0B2 have provided insights into its interaction interfaces, aiding in the design of small molecules targeting metabolic syndromes. Additionally, it serves as a tool to study epigenetic modifications and nuclear receptor crosstalk, furthering understanding of metabolic and hormonal signaling networks.
In summary, NR0B2 recombinant protein is a pivotal resource for decoding its regulatory functions and developing therapeutic strategies for metabolic and neoplastic diseases.
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