| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MID1 |
| Uniprot No | O15344 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-667aa |
| 氨基酸序列 | METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNESVES ITAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET RRERAFDANT MTSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVM RLRKLAQQIA NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT VHWTSDDEFS VVSYELQYTI FTGQANVVSL CNSADSWMIV PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR HYWEVVISGS TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH LRRVGILLDY DNGSIAFYDA LNSIHLYTFD VAFAQPVCPT FTVWNKCLTI ITGLPIPDHL DCTEQLP |
| 预测分子量 | 75,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MID1重组蛋白的3篇参考文献示例(部分信息可能为虚构或简化，仅供参考)：
1. **文献名称**：*MID1 Recombinant Protein Binds Microtubules and Regulates PP2A Activity*
**作者**：Liu Y, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组MID1蛋白，发现其通过N端结构域与微管结合，并通过C端结构域招募蛋白磷酸酶PP2A。实验表明MID1的泛素连接酶活性依赖于微管网络完整性。
2. **文献名称**：*Structural Characterization of the MID1-α4 Complex Using Recombinant Proteins*
**作者**：Schneider T, et al.
**摘要**：通过共表达技术获得MID1与α4蛋白的复合物重组蛋白，X射线晶体学分析揭示了两者结合的分子界面。该复合物结构解释了MID1突变导致Opitz综合征的潜在分子机制。
3. **文献名称**：*In Vitro Ubiquitination Assay of MID1 Recombinant Mutants*
**作者**：García-Castro M, et al.
**摘要**：研究构建了多个MID1疾病相关突变体的重组蛋白，通过体外泛素化实验发现RING结构域突变会显著降低其E3连接酶活性，提示功能丧失是Opitz综合征的关键致病因素。
注：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science以“MID1 recombinant protein”为关键词检索最新研究。
**Background of MID1 Recombinant Protein**
The MID1 (Midline-1) gene encodes a microtubule-associated protein belonging to the tripartite motif (TRIM) family, characterized by its RING finger, B-box, and coiled-coil domains. Initially identified for its role in X-linked Opitz G/BBB syndrome—a genetic disorder causing midline developmental defects—MID1 functions as an E3 ubiquitin ligase, regulating protein turnover via the ubiquitin-proteasome system. It forms multiprotein complexes with PP2A phosphatase and other partners, influencing cellular processes like cytoskeletal organization, mRNA translation, and cell proliferation.
Recombinant MID1 protein is produced *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and pathological mechanisms. Its truncated or mutated variants, linked to disease, help dissect functional domains and dysregulation pathways. For instance, aberrant MID1 activity disrupts mTOR signaling, contributing to tumorigenesis or neurodevelopmental defects.
Research on MID1 recombinant protein also aids in drug screening, particularly for disorders tied to ubiquitination or PP2A dysfunction. Despite progress, challenges remain in resolving its full 3D structure and context-dependent regulatory roles. Current studies focus on its post-translational modifications and tissue-specific interactions, aiming to develop targeted therapies for MID1-associated diseases.
(Word count: ~200; remaining space allows expansion on specific applications or mechanisms if needed.)
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