| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gp210 |
| Uniprot No | Q8TEM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-238aa |
| 氨基酸序列 | LNIPKVLLPFTRATRVNFTLEASEGCYRWLSTRPEVASIEPLGLDEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHDIQIVSTTRELYLEDSPLELKIQALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSGMKTGSSKLKARIQEAVYKNVRPAEVRLL |
| 预测分子量 | 27.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于gp210重组蛋白的模拟参考文献示例，基于常见研究方向概括：
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1. **《Autoantigen gp210 recombinant protein enables sensitive detection of primary biliary cholangitis antibodies》**
- 作者：Y. Muro, M. Sugiura
- 摘要：研究利用重组gp210蛋白建立新型ELISA检测方法，用于原发性胆汁性肝硬化(PBC)患者血清中自身抗体的高效识别，验证其诊断灵敏度和特异性。
2. **《Expression and epitope mapping of human gp210 in Escherichia coli》**
- 作者：S. Cozar, E. Cabezas
- 摘要：报道人源gp210蛋白在大肠杆菌中的重组表达策略，通过片段筛选定位其抗原表位，为开发PBC诊断工具提供基础数据。
3. **《Structural insights into gp210’s role in nuclear pore assembly》**
- 作者：T. Tsimikas, J. Ellenberg
- 摘要：利用重组gp210蛋白结合冷冻电镜技术解析其参与核孔复合体组装的分子机制，揭示C端结构域对核膜锚定的关键作用。
4. **《Recombinant gp210 improves early diagnosis of autoimmune liver diseases》**
- 作者：L. Liu et al.
- 摘要：评估重组gp210蛋白在多种自身免疫性肝病中的诊断价值，发现其对PBC早期筛查具有高阳性预测价值，优于传统免疫荧光法。
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**说明**：以上文献为模拟示例，反映gp210重组蛋白在自身免疫疾病诊断、蛋白表达及结构功能领域的典型研究。实际文献需通过学术数据库(如PubMed)检索确认。
**Background of gp210 Recombinant Protein**
The gp210 (glycoprotein 210) recombinant protein is derived from the human *GP210* gene, which encodes a transmembrane protein integral to the nuclear pore complex (NPC). The NPC is a large structure embedded in the nuclear envelope, regulating bidirectional transport of molecules between the nucleus and cytoplasm. gp210. with a molecular weight of approximately 210 kDa, is one of the core components of the NPC, anchoring it to the nuclear membrane via its C-terminal transmembrane domain. Its extracellular N-terminal domain contains multiple O-linked glycosylation sites, contributing to its functional stability.
gp210 gained significant attention due to its association with autoimmune diseases, particularly primary biliary cholangitis (PBC). In PBC, autoantibodies targeting gp210 are detected in ~20-30% of patients, serving as a diagnostic and prognostic biomarker. These antibodies correlate with aggressive disease progression, highlighting gp210’s role in autoimmune pathogenesis.
Recombinant gp210 protein is produced using biotechnological methods, such as expression in mammalian or bacterial systems, followed by purification. This engineered protein retains key epitopes for antibody recognition, making it valuable for research and clinical applications. It is widely used in ELISA-based assays to detect anti-gp210 antibodies in patient sera, aiding PBC diagnosis. Additionally, it serves as a tool to study NPC assembly, nucleocytoplasmic transport mechanisms, and autoimmune responses.
Studies also explore gp210’s potential in therapeutic target discovery, particularly for modulating NPC dysfunction or autoimmune activity. Its recombinant form thus bridges basic research and translational medicine, offering insights into cellular biology and autoimmune disease management.
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