| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCAR3 |
| Uniprot No | O75815 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-825aa |
| 氨基酸序列 | MAAGKFASLPRNMPVNHQFPLASSMDLLSSRSPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHMGTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKELEEELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTKGRPDVAKRLSLTMGGVQAREQNLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPPQSSGVDTSPCPNSPVFRTGSEPALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPSAWLNSEANYCELNPAFATGCGRGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLETVSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSSGLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALMKALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQAVTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSKGAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL |
| 预测分子量 | 92,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCAR3重组蛋白的3篇文献参考及简要摘要：
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1. **文献名称**：*BCAR3 regulates Src/p130Cas association, cell proliferation, and migration in transformed cells*
**作者**：Cabodi, S. et al.
**摘要**：
该研究通过表达重组BCAR3蛋白，揭示了其与Src激酶和p130Cas的相互作用机制，发现BCAR3通过调控黏着斑复合体促进细胞增殖和迁移，提示其在癌症转移中的作用。
2. **文献名称**：*Structural and functional analysis of the BCAR3-AND-34 complex in cell signaling*
**作者**：Lu, Y. et al.
**摘要**：
利用重组BCAR3蛋白进行结构解析和体外结合实验，阐明了BCAR3与AND-34形成的复合物如何激活Rho家族GTPases，进而影响细胞骨架重塑和侵袭性表型。
3. **文献名称**：*BCAR3 drives resistance to estrogen receptor antagonists through ERK/MAPK activation*
**作者**：Zhang, X. et al.
**摘要**：
研究发现，重组BCAR3蛋白的过表达通过激活ERK/MAPK信号通路，导致乳腺癌细胞对雌激素受体拮抗剂(如他莫昔芬)产生耐药性，提示其作为治疗靶点的潜力。
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**注**：若需获取具体文献来源(期刊、年份等)，建议通过PubMed或Google Scholar检索标题或作者进一步确认。
**Background of BCAR3 Recombinant Protein**
BCAR3 (Breast Cancer Anti-estrogen Resistance 3) is a signaling adaptor protein implicated in cellular processes such as cell migration, proliferation, and resistance to anti-cancer therapies. Initially identified for its role in conferring resistance to anti-estrogen therapies in breast cancer cells, BCAR3 interacts with proteins like BCAR1/p130Cas to regulate integrin and growth factor signaling pathways. Structurally, it contains a conserved SH2 domain and a GAP-like domain, though it lacks canonical GTPase-activating protein (GAP) activity. Instead, BCAR3 modulates cytoskeletal dynamics and cell adhesion by scaffolding signaling complexes, particularly those involving RAP1 and RAC1 GTPases.
Recombinant BCAR3 protein is produced using biotechnological methods, often in *E. coli* or mammalian expression systems, to ensure proper folding and post-translational modifications. This engineered protein serves as a critical tool for *in vitro* studies, enabling researchers to dissect its molecular interactions, structural motifs, and functional roles in cancer progression and metastasis. Studies using recombinant BCAR3 have highlighted its involvement in tumor cell invasion, survival under therapeutic stress, and cross-talk between oncogenic pathways like EGFR and HER2.
Beyond cancer, BCAR3 is linked to physiological processes such as angiogenesis and immune cell regulation. Its recombinant form is also explored for drug discovery, aiming to develop inhibitors that disrupt BCAR3-mediated signaling nodes in resistant cancers. Overall, BCAR3 recombinant protein remains a vital resource for unraveling mechanisms of therapy resistance and identifying novel targets in precision oncology.
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