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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANG-3 |
| Uniprot No | O95841 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-491aa |
| 氨基酸序列 | GQFKIKK INQRRYPRAT DGKEEAKKCA YTFLVPEQRI TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID VLQLVVDVDG NIVNEVKLLR KESRNMNSRV TQLYMQLLHE IIRKRDNSLE LSQLENKILN VTTEMLKMAT RYRELEVKYA SLTDLVNNQS VMITLLEEQC LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ RDPGYPRDLM PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW LGLENIYMLS NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG TYQGNAGDSM MWHNGKQFTT LDRDKDMYAG NCAHFHKGGW WYNACAHSNL NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL RAVQMMIKPI D |
| 预测分子量 | 56,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANG-3重组蛋白的模拟参考文献示例，供参考。建议通过学术数据库(如PubMed、Google Scholar)进一步验证和获取详细信息：
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1. **文献名称**: *Recombinant Angiopoietin-3 Promotes Vascular Stabilization in Experimental Models*
**作者**: Kim S, et al.
**摘要**: 本研究通过大肠杆菌表达系统制备重组ANG-3蛋白，发现其能通过激活Tie2受体增强血管内皮细胞稳定性，并在小鼠缺血模型中减少血管渗漏，提示其在治疗血管相关疾病中的潜力。
2. **文献名称**: *Structural Insights into ANG-3/Tie2 Interaction by Cryo-EM*
**作者**: Zhang L, et al.
**摘要**: 通过冷冻电镜解析ANG-3重组蛋白与Tie2受体复合物的三维结构，揭示了其独特的结合域，为设计靶向血管生成的药物提供了结构基础。
3. **文献名称**: *ANG-3 Recombinant Protein Attenuates Tumor Angiogenesis in Colorectal Cancer*
**作者**: Chen W, et al.
**摘要**: 在结直肠癌小鼠模型中，ANG-3重组蛋白显著抑制肿瘤血管异常增生，并增强化疗效果，表明其作为抗血管生成治疗剂的潜在应用。
4. **文献名称**: *Optimization of ANG-3 Production in Mammalian Cell Culture*
**作者**: Wang Y, et al.
**摘要**: 通过CHO细胞系统优化ANG-3重组蛋白的表达和糖基化修饰，提高了蛋白产量和生物活性，为大规模生产奠定基础。
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**注意**：以上文献为模拟示例，实际研究可能需要结合具体物种(如ANG-3可能指小鼠蛋白，对应人类中的ANGPT4)进行检索。建议使用关键词“ANG-3 recombinant protein”“Angiopoietin-3”“ANGPT3/ANGPT4”等扩展查询。
**Background of ANG-3 Recombinant Protein**
ANG-3 (angiogenin-3), a member of the ribonuclease (RNase) A superfamily, was initially identified for its structural homology to angiogenin (ANG), a protein critical in angiogenesis and cell survival. Discovered in the early 2000s, ANG-3 shares conserved catalytic residues characteristic of RNases but exhibits distinct biological activities. Unlike ANG, which is widely studied for its role in promoting blood vessel formation and neuroprotection, ANG-3 has been less characterized, with research suggesting potential differences in tissue expression and functional mechanisms.
ANG-3 is encoded by the *ANGPT3* gene and is primarily expressed in the liver and certain epithelial tissues. Recombinant ANG-3 protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to ensure high purity and bioactivity. Its recombinant form enables studies on structure-function relationships, particularly its weak ribonucleolytic activity compared to other angiogenins. Despite low enzymatic activity, ANG-3 may interact with cellular receptors like integrins or extracellular matrix components, modulating pathways involved in cell migration, inflammation, or tissue repair.
Emerging evidence hints at ANG-3’s dual role in both promoting and inhibiting angiogenesis, depending on the cellular context. It has been implicated in tumor progression, ischemic diseases, and inflammatory conditions, though mechanistic insights remain limited. Current research focuses on elucidating its signaling networks, including potential crosstalk with VEGF or NF-κB pathways, and exploring therapeutic applications in cancer, wound healing, or neurodegenerative disorders. However, challenges persist in defining its precise physiological roles and clinical relevance, necessitating further in vivo and translational studies.
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