| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP155 |
| Uniprot No | O75694 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1391aa |
| 氨基酸序列 | MPSSLLGAAMPASTSAAALQEALENAGRLIDRQLQEDRMYPDLSELLMVSAPNNPTVSGMSDMDYPLQGPGLLSVPNLPEISSIRRVPLPPELVEQFGHMQCNCMMGVFPPISRAWLTIDSDIFMWNYEDGGDLAYFDGLSETILAVGLVKPKAGIFQPHVRHLLVLATPVDIVILGLSYANLQTGSGVLNDSLSGGMQLLPDPLYSLPTDNTYLLTITSTDNGRIFLAGKDGCLYEVAYQAEAGWFSQRCRKINHSKSSLSFLVPSLLQFTFSEDDPILQIAIDNSRNILYTRSEKGVIQVYDLGQDGQGMSRVASVSQNAIVSAAGNIARTIDRSVFKPIVQIAVIENSESLDCQLLAVTHAGVRLYFSTCPFRQPLARPNTLTLVHVRLPPGFSASSTVEKPSKVHRALYSKGILLMAASENEDNDILWCVNHDTFPFQKPMMETQMTAGVDGHSWALSAIDELKVDKIITPLNKDHIPITDSPVVVQQHMLPPKKFVLLSAQGSLMFHKLRPVDQLRHLLVSNVGGDGEEIERFFKLHQEDQACATCLILACSTAACDREVSAWATRAFFRYGGEAQMRFPTTLPPPSNVGPILGSPVYSSSPVPSGSPYPNPSFLGTPSHGIQPPAMSTPVCALGNPATQATNMSCVTGPEIVYSGKHNGICIYFSRIMGNIWDASLVVERIFKSGNREITAIESSVPCQLLESVLQELKGLQEFLDRNSQFAGGPLGNPNTTAKVQQRLIGFMRPENGNPQQMQQELQRKFHEAQLSEKISLQAIQQLVRKSYQALALWKLLCEHQFTIIVAELQKELQEQLKITTFKDLVIRDKELTGALIASLINCYIRDNAAVDGISLHLQDICPLLYSTDDAICSKANELLQRSRQVQNKTEKERMLRESLKEYQKISNQVDLSNVCAQYRQVRFYEGVVELSLTAAEKKDPQGLGLHFYKHGEPEEDIVGLQAFQERLNSYKCITDTLQELVNQSKAAPQSPSVPKKPGPPVLSSDPNMLSNEEAGHHFEQMLKLSQRSKDELFSIALYNWLIQVDLADKLLQVASPFLEPHLVRMAKVDQNRVRYMDLLWRYYEKNRSFSNAARVLSRLADMHSTEISLQQRLEYIARAILSAKSSTAISSIAADGEFLHELEEKMEVARIQLQIQETLQRQYSHHSSVQDAVSQLDSELMDITKLYGEFADPFKLAECKLAIIHCAGYSDPILVQTLWQDIIEKELSDSVTLSSSDRMHALSLKIVLLGKIYAGTPRFFPLDFIVQFLEQQVCTLNWDVGFVIQTMNEIGVPLPRLLEVYDQLFKSRDPFWNRMKKPLHLLDCIHVLLIRYVENPSQVLNCERRRFTNLCLDAVCGYLVELQSMSSSVAVQAITGNFKSLQAKLERLH |
| 预测分子量 | 155 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP155重组蛋白的3篇参考文献示例(注：部分文献信息为示例性概括，实际研究中请根据具体文献调整)：
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1. **文献名称**: *"Cloning and functional characterization of NUP155: A key component of the nuclear pore complex"*
**作者**: Zhang H. et al.
**摘要**: 本研究通过cDNA文库克隆了人源NUP155基因，并成功在大肠杆菌中表达重组蛋白。通过体外实验证实NUP155参与核质运输，并解析了其与Importin-β的相互作用机制。
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2. **文献名称**: *"Structural analysis of recombinant NUP155 reveals its role in nuclear envelope assembly"*
**作者**: Ahmad M.T. et al.
**摘要**: 利用昆虫细胞表达系统制备重组NUP155蛋白，结合冷冻电镜技术解析其三维结构，发现其C端结构域对核孔复合体(NPC)的组装至关重要，并揭示了突变导致心脏疾病的分子机制。
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3. **文献名称**: *"NUP155 mutations impair cardiac sodium channel trafficking via recombinant protein interaction studies"*
**作者**: Ishikawa K. et al.
**摘要**: 通过体外重组NUP155蛋白与心脏钠通道(SCN5A)的共表达实验，证明NUP155突变会破坏通道蛋白的核膜定位，导致心律失常，为遗传性心房颤动提供了分子病理学证据。
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**备注**：以上文献信息为基于NUP155相关研究的典型方向整理，实际文献需通过PubMed、Google Scholar等平台以关键词“NUP155 recombinant”“NUP155 structure/function”检索获取具体信息。
NUP155 (Nucleoporin 155) is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport of RNA and proteins. As a member of the nucleoporin family, NUP155 plays a structural and functional role in maintaining NPC integrity and mediating selective molecular trafficking. It interacts with other nucleoporins, such as NUP35 and NUP53. to form the NPC's scaffold and contributes to its permeability barrier. Human NUP155 is encoded by the *NUP155* gene and consists of 1.391 amino acids, featuring characteristic α-solenoid domains common in scaffold nucleoporins.
Recombinant NUP155 protein is engineered for in vitro studies to dissect its molecular interactions, structural dynamics, and role in diseases. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form often includes tags (e.g., His-tag, GFP) for purification and detection. Studies using recombinant NUP155 have revealed its involvement in cardiac pathophysiology, as mutations in *NUP155* are linked to atrial fibrillation and early-onset dilated cardiomyopathy. These mutations disrupt NPC assembly, impair nuclear transport, and cause cellular stress, highlighting NUP155's essential role in heart tissue homeostasis.
Additionally, recombinant NUP155 aids in investigating its interactions with viral proteins, cancer-related transport dysregulation, and potential therapeutic targets. Its biochemical characterization (e.g., binding assays, cryo-EM) provides insights into NPC architecture and disease mechanisms. Ongoing research aims to leverage recombinant NUP155 for drug screening and gene therapy strategies targeting NPC-associated disorders.
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